PMID- 20522956
OWN - NLM
STAT- MEDLINE
DCOM- 20110110
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 33
IP  - 6
DP  - 2010
TI  - Proteoglycans regulate the chemotaxis of dendritic cells derived from human 
      peripheral blood monocytes.
PG  - 938-44
AB  - Dendritic cells (DCs) are a type of antigen-presenting cell which play an 
      essential role in the immune system. The transition from immature DC (iDCs) to 
      mature DCs (mDCs) requires appropriate maturation stimuli, such as 
      pro-inflammatory cytokines or pathogen-derived components. Proteoglycans (PGs), 
      which are composed of core proteins and the glycosaminoglycans that bind to them, 
      are one of the main components of the extracellular matrix around pathogens such 
      as bacteria. This study investigated the effects of PG extracted from the nasal 
      septum cartilage of whale (W-PG) on the maturation of DCs derived from human 
      peripheral blood monocytes. iDCs were prepared from human monocytes using 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 
      (IL-4). The iDCs were stimulated by W-PG alone. In another type of experiment, 
      the iDCs were stimulated by MIX (tumor necrosis factor-alpha (TNF-alpha), 
      IL-1beta, IL-6 and prostaglandin E(2) (PGE(2))) or a combination of MIX plus 
      W-PG. The stimulation of W-PG alone did not induce the phenotypic maturation from 
      iDCs. However, W-PG promoted the up-regulation of chemokine receptor CCR7-surface 
      expression and the chemotactic responsiveness to CCR7 ligand macrophage 
      inflammatory protein-3beta on MIX-stimulated mDCs although W-PG did not influence 
      matrix metalloproteinase-9 activity which is an important factor in DC migration 
      through the extracellular matrix. The findings that W-PG can selectively regulate 
      the chemotactic activity of DCs in vitro under inflammatory conditions therefore 
      indicate that the interaction of PGs with immune cells including DCs plays an 
      important role in the immune response under the milieu of innate immunity.
FAU - Yoshino, Hironori
AU  - Yoshino H
AD  - Department of Radiological Life Sciences, Division of Medical Life Sciences, 
      Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 
      036-8564, Japan.
FAU - Takahashi, Kenji
AU  - Takahashi K
FAU - Monzen, Satoru
AU  - Monzen S
FAU - Kashiwakura, Ikuo
AU  - Kashiwakura I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Cytokines)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Proteoglycans)
RN  - 0 (Receptors, CCR7)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Cartilage
MH  - Cell Differentiation/*drug effects
MH  - Chemotaxis/*drug effects
MH  - Cytokines/pharmacology
MH  - Dendritic Cells/cytology/*drug effects/metabolism
MH  - Dinoprostone/pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor
MH  - Humans
MH  - Inflammation/metabolism
MH  - Interleukin-4
MH  - Macrophage Inflammatory Proteins/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Monocytes/*cytology
MH  - Nasal Septum
MH  - Proteoglycans/*pharmacology
MH  - Receptors, CCR7/metabolism
MH  - Up-Regulation
MH  - Whales
EDAT- 2010/06/05 06:00
MHDA- 2011/01/11 06:00
CRDT- 2010/06/05 06:00
PHST- 2010/06/05 06:00 [entrez]
PHST- 2010/06/05 06:00 [pubmed]
PHST- 2011/01/11 06:00 [medline]
AID - JST.JSTAGE/bpb/33.938 [pii]
AID - 10.1248/bpb.33.938 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2010;33(6):938-44. doi: 10.1248/bpb.33.938.