PMID- 20524194
OWN - NLM
STAT- MEDLINE
DCOM- 20100909
LR  - 20200930
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 94
IP  - 1
DP  - 2010 Jul
TI  - Synthesis and anticoagulant activity of heparin immobilized "end-on" to 
      polystyrene microspheres coated with end-group activated polyethylene oxide.
PG  - 187-95
LID - 10.1002/jbm.b.31640 [doi]
AB  - Thiol groups were introduced to unfractionated heparin (UFH) and end-aminated 
      heparin (HepNH(2)) by reaction with 2-iminothiolane under conditions favoring 
      selective modification of terminal over primary amines. End-thiolated heparin 
      retained anticoagulant activity as shown by the activated partial thromboplastin 
      time (aPTT) and anti-Factor Xa (anti-FXa) assays. Thiolated heparins were linked 
      to pyridyl-disulfide activated poly(ethylene oxide)-poly(propylene 
      oxide)-poly(ethylene oxide) triblock copolymers adsorbed to 1.15-mum polystyrene 
      microspheres. Surface loadings were similarly low for each type of thiolated 
      heparin. No anticoagulant activity was observed with aPTT assays of heparinized 
      microspheres, due either to the presence of an insufficient amount of immobilized 
      heparin, or to steric constraints inhibiting the formation of a functional 
      heparin-antihrombin complex. However, immobilized heparin retained substantial 
      anti-FXa activity, with significantly greater activity exhibited by the 
      end-thiolated HepNH(2) than the internally (randomly) thiolated UFH.
CI  - (c) 2010 Wiley Periodicals, Inc.
FAU - Fry, Allyson K
AU  - Fry AK
AD  - School of Chemical, Biological and Environmental Engineering, Oregon State 
      University, Corvallis, Oregon 97331, USA.
FAU - Schilke, Karl F
AU  - Schilke KF
FAU - McGuire, Joseph
AU  - McGuire J
FAU - Bird, Karyn E
AU  - Bird KE
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Amines)
RN  - 0 (Anticoagulants)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Factor Xa Inhibitors)
RN  - 0 (PEO-PPO-PEO)
RN  - 0 (Polystyrenes)
RN  - 0 (Propylene Glycols)
RN  - 0 (Sulfhydryl Compounds)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Adsorption
MH  - Amines/chemistry
MH  - *Anticoagulants/chemistry/pharmacology
MH  - Biocompatible Materials/chemistry/pharmacology
MH  - Blood Coagulation/*drug effects
MH  - Factor Xa Inhibitors
MH  - *Heparin/chemistry/pharmacology
MH  - Humans
MH  - Materials Testing
MH  - *Microspheres
MH  - Molecular Structure
MH  - Partial Thromboplastin Time
MH  - Polyethylene Glycols/*chemistry
MH  - Polystyrenes/*chemistry
MH  - Propylene Glycols/chemistry
MH  - Sulfhydryl Compounds/chemistry
EDAT- 2010/06/05 06:00
MHDA- 2010/09/10 06:00
CRDT- 2010/06/05 06:00
PHST- 2010/06/05 06:00 [entrez]
PHST- 2010/06/05 06:00 [pubmed]
PHST- 2010/09/10 06:00 [medline]
AID - 10.1002/jbm.b.31640 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2010 Jul;94(1):187-95. doi: 
      10.1002/jbm.b.31640.