PMID- 20525429
OWN - NLM
STAT- MEDLINE
DCOM- 20110412
LR  - 20181201
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 19
IP  - 10
DP  - 2010
TI  - Nonsenescent Hsp27-upregulated MSCs implantation promotes neuroplasticity in 
      stroke model.
PG  - 1261-79
LID - 10.3727/096368910X507204 [doi]
AB  - Cellular senescence induces changes in cellular physiology, morphology, 
      proliferative capacity, and gene expression. Stem cell senescence might be one of 
      the major issues of limited efficacy of stem cell transplantation. In this study, 
      we demonstrated that implantation of human umbilical cord mesenchymal stem cells 
      (hUCMSCs) cultured in human umbilical cord serum (hUCS) significantly enhanced 
      neuroplasticity and angiogenesis in stroke and ischemic limb models. 
      Immunophenotypic analysis indicated that hUCMSCs cultured in hUCS had more small 
      and rapidly self-renewing cells than those expanded in FCS. The main cause of 
      greater senescence in FCS-cultured cells was increased generation of reactive 
      oxygen species (ROS). Proteome profiling showed significantly more 
      senescence-associated vimentin in FCS-cultured hUCMSCs than in hUCS-cultured 
      hUCMSCs. In contrast, there was significant upregulation of heat shock protein 27 
      (Hsp27) in the hUCS-cultured hUCMSCs. By gene targeting, we found that 
      overexpression of Hsp27 may downregulate vimentin expression through inhibition 
      of the nuclear translocation of p65 (NF-kappaB signaling). Thus, an interaction 
      between Hsp27 and vimentin may modulate the degree of senescence in hUCS- and 
      FCS-cultured hUCMSCs. In summary, hUCMSCs exhibiting senescence are detrimental 
      to cell engraftment and differentiation in animal models via activation of NF-kappaB 
      pathway. Human stem cells incubated in hUCS might reduce the senescent process 
      through upregulation of Hsp27 to increase implantation efficiency.
FAU - Liu, Shih-Ping
AU  - Liu SP
AD  - Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan.
FAU - Ding, Dah-Ching
AU  - Ding DC
FAU - Wang, Hsiao-Jung
AU  - Wang HJ
FAU - Su, Ching-Yuan
AU  - Su CY
FAU - Lin, Shinn-Zong
AU  - Lin SZ
FAU - Li, Hung
AU  - Li H
FAU - Shyu, Woei-Cherng
AU  - Shyu WC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100603
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (HSP27 Heat-Shock Proteins)
RN  - 0 (Proteome)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Vimentin)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Fetal Blood/physiology
MH  - HSP27 Heat-Shock Proteins/*metabolism
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - *Neuronal Plasticity
MH  - Phenotype
MH  - Proteome/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Stroke/*therapy
MH  - Transcription Factor RelA/metabolism
MH  - Umbilical Cord/cytology
MH  - Up-Regulation
MH  - Vimentin/metabolism
EDAT- 2010/06/08 06:00
MHDA- 2011/04/13 06:00
CRDT- 2010/06/08 06:00
PHST- 2010/06/08 06:00 [entrez]
PHST- 2010/06/08 06:00 [pubmed]
PHST- 2011/04/13 06:00 [medline]
AID - ct0034liu [pii]
AID - 10.3727/096368910X507204 [doi]
PST - ppublish
SO  - Cell Transplant. 2010;19(10):1261-79. doi: 10.3727/096368910X507204. Epub 2010 
      Jun 3.