PMID- 20527186
OWN - NLM
STAT- MEDLINE
DCOM- 20110329
LR  - 20161018
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 31
IP  - 4
DP  - 2010 Apr
TI  - [Formation and reaction mechanism of simultaneous nitrogen and phosphorus removal 
      by aerobic granular sludge].
PG  - 1021-9
AB  - Aerobic granular sludge (AGS) for simultaneous nitrogen and phosphorus removal 
      (SNPR) was cultivated and studied in two lab-scale sequencing batch reactors 
      (named as A & B) treating real domestic wastewater, additional carbon (sodium 
      propionate and sodium acetate for A, glucose for B) was added to make the ratio 
      of COD:N:P as 360:60:6, good SNPR was achieved at normal (18-27 degrees C) and 
      low temperature (9-13 degrees C). The microbial community composition and 
      distribution, distribution of cells and extracellular polymeric substances (EPS) 
      and morphologies of the AGS were investigated using fluorescence in situ 
      hybridization (FISH), in situ fluorescent staining and scanning electron 
      microscope (SEM), respectively. FISH results showed that ammonium-oxidizing 
      bacteria comprised 12% of all the bacteria and were mainly located at the outer 
      parts of the granules; phosphates accumulating organisms comprised 40% of all the 
      bacteria and were mainly located in the inner parts of the granules. 
      Nitrification was the rate controlling step; denitrifying phosphate accumulating 
      organisms inside the granular sludge might be responsible for denitrification in 
      the aerobic phase, which enabled effective SNPR. Live/dead fluorescent staining 
      results showed that dead cells were distributed throughout the granules and live 
      cells were principally distribution of polysaccharide (including 
      alpha-mannopyranosyl, alpha-glucopyranosyl sugars and beta-D-glucopyranose 
      polysaccharides) of EPS in AGS were influenced by different carbon sources, but 
      the contents and distributions of protein and lipids were not, the contents of 
      protein was the largest. Polysaccharide was responsible for the formation and 
      maintenance of aerobic granular sludge. SEM results showed that bacilli and cocci 
      were the main bacteria in the granules of A and B, respectively. Carbon sources 
      affected the bacteria type and the SNPR efficiency, sodium propionate and sodium 
      acetate were better than glucose.
FAU - Gao, Jing-feng
AU  - Gao JF
AD  - College of Environmental and Energy Engineering, Beijing University of 
      Technology, Beijing 100124, China. gao.jingfeng@bjut.edu.cn
FAU - Chen, Ran-ni
AU  - Chen RN
FAU - Su, Kai
AU  - Su K
FAU - Zhang, Qian
AU  - Zhang Q
FAU - Peng, Yong-zhen
AU  - Peng YZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Aerobiosis
MH  - Bacteria/metabolism
MH  - Biodegradation, Environmental
MH  - Bioreactors/microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrogen/*isolation & purification/metabolism
MH  - Particle Size
MH  - Phosphorus/*isolation & purification/metabolism
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid/*methods
EDAT- 2010/06/10 06:00
MHDA- 2011/03/30 06:00
CRDT- 2010/06/10 06:00
PHST- 2010/06/10 06:00 [entrez]
PHST- 2010/06/10 06:00 [pubmed]
PHST- 2011/03/30 06:00 [medline]
PST - ppublish
SO  - Huan Jing Ke Xue. 2010 Apr;31(4):1021-9.