PMID- 2052746
OWN - NLM
STAT- MEDLINE
DCOM- 19910723
LR  - 20131121
IS  - 0034-5164 (Print)
IS  - 0034-5164 (Linking)
VI  - 72
IP  - 1
DP  - 1991 Apr
TI  - Substrate dependence of human neutrophil protein kinase C.
PG  - 13-25
AB  - To determine the contribution of phosphate acceptor substrate to the pattern of 
      activity of calcium-dependent, phospholipid-sensitive protein kinase (protein 
      kinase C, PKC), we assayed cytosolic and particulate PKC activity for histone, 
      troponin, myosin light chain (MLC), and endogenous cellular proteins in human 
      neutrophils stimulated with phorbol myristate acetate (PMA), the chemotactic 
      peptide n-formyl-methionyl-leucyl-phenylalanine (FMLP) and synergistic 
      stimulation with both agonists. In general, phosphotransferase activity in 
      neutrophil subfractions toward troponin and endogenous proteins paralleled that 
      toward histone, but MLC was a poor substrate for PKC and the pattern of 
      phosphotransferase activity differed from that seen with the other substrates. 
      Furthermore, the phosphorylation of endogenous neutrophil cytosolic proteins 
      increased significantly after stimulation with FMLP, suggesting an endogenous 
      cytosolic substrate(s) which increased in concentration following stimulation. We 
      conclude that histone is a useful phosphate acceptor for study of PKC activity in 
      human neutrophils, but substrate variability occurs and may influence 
      interpretation of results in assays of PKC activity.
FAU - Gay, J C
AU  - Gay JC
AD  - Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN.
FAU - Stitt, E S
AU  - Stitt ES
LA  - eng
GR  - AI 22383-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Res Commun Chem Pathol Pharmacol
JT  - Research communications in chemical pathology and pharmacology
JID - 0244734
RN  - 0 (Histones)
RN  - 0 (Troponin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.6.4.1 (Myosins)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Histones/metabolism
MH  - Humans
MH  - Myosins/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*enzymology
MH  - Phosphorylation
MH  - Protein Kinase C/*blood
MH  - Substrate Specificity
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Troponin/metabolism
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
PST - ppublish
SO  - Res Commun Chem Pathol Pharmacol. 1991 Apr;72(1):13-25.