PMID- 20528801
OWN - NLM
STAT- MEDLINE
DCOM- 20110211
LR  - 20211203
IS  - 1748-1716 (Electronic)
IS  - 1748-1708 (Linking)
VI  - 200
IP  - 3
DP  - 2010 Nov
TI  - Influence of supplementation with branched-chain amino acids in combination with 
      resistance exercise on p70S6 kinase phosphorylation in resting and exercising 
      human skeletal muscle.
PG  - 237-48
LID - 10.1111/j.1748-1708.2010.02151.x [doi]
AB  - AIM: Skeletal muscle growth is thought to be regulated by the mammalian target of 
      rapamycin (mTOR) pathway, which can be activated by resistance exercise and 
      branched-chain amino acids (BCAA). The major aim of the present study was to 
      distinguish between the influence of resistance exercise and BCAA on key enzymes 
      considered to be involved in the regulation of protein synthesis, including 
      p70(S6) kinase (p70(S6k)). METHODS: Nine healthy subjects (four men and five 
      women) performed unilateral resistance exercise on two occasions separated by 1 
      month. Subjects were randomly supplied either a mixture of BCAA or flavoured 
      water. Muscle biopsies were taken from both resting and exercising muscle before, 
      after and 1 h after exercise. RESULTS: Phosphorylation of Akt was unaltered by 
      either resistance exercise and/or BCAA supplementation whereas mTOR 
      phosphorylation was enhanced (P<0.05) to a similar extent in both exercising and 
      resting muscle following exercise in the absence (70-90%) and presence of BCAA 
      supplementation (80-130%). Phosphorylation of p70(S6k) was unaffected by 
      resistance exercise alone; however, BCAA intake increased (P<0.05) this 
      phosphorylation in both legs following exercise. In resting muscle, a 5- and 
      16-fold increase in p70(S6k) was observed immediately after and 1 h after 
      exercise, respectively, as compared to 11- and 30-fold increases in the 
      exercising muscle. Phosphorylation of eukaryotic elongation factor 2 was 
      attenuated 1 h after exercise (P<0.05) in both resting (10-40%) and exercising 
      muscle (30-50%) under both conditions. CONCLUSION: The present findings indicate 
      that resistance exercise and BCAA exert both separate and combined effects on the 
      p70(S6k) phosphorylation in an Akt-independent manner.
CI  - (c) 2010 The Authors. Journal compilation (c) 2010 Scandinavian Physiological 
      Society.
FAU - Apro, W
AU  - Apro W
AD  - Astrand Laboratory, Swedish School of Sport and Health Sciences, Stockholm, 
      Sweden.
FAU - Blomstrand, E
AU  - Blomstrand E
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Acta Physiol (Oxf)
JT  - Acta physiologica (Oxford, England)
JID - 101262545
RN  - 0 (Amino Acids, Branched-Chain)
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Eukaryotic Initiation Factor-2)
RN  - 0 (Insulin)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adult
MH  - Amino Acids, Branched-Chain/*administration & dosage/blood
MH  - Biomarkers/blood
MH  - Biopsy
MH  - Blood Glucose/metabolism
MH  - *Dietary Supplements
MH  - Eukaryotic Initiation Factor-2/metabolism
MH  - Female
MH  - Humans
MH  - Insulin/blood
MH  - Lactic Acid/blood
MH  - Male
MH  - *Muscle Contraction
MH  - Muscle, Skeletal/*drug effects/enzymology
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - *Resistance Training
MH  - Ribosomal Protein S6 Kinases, 70-kDa/*metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Time Factors
MH  - Young Adult
EDAT- 2010/06/10 06:00
MHDA- 2011/02/12 06:00
CRDT- 2010/06/10 06:00
PHST- 2010/06/10 06:00 [entrez]
PHST- 2010/06/10 06:00 [pubmed]
PHST- 2011/02/12 06:00 [medline]
AID - APS2151 [pii]
AID - 10.1111/j.1748-1708.2010.02151.x [doi]
PST - ppublish
SO  - Acta Physiol (Oxf). 2010 Nov;200(3):237-48. doi: 
      10.1111/j.1748-1708.2010.02151.x.