PMID- 20530791
OWN - NLM
STAT- MEDLINE
DCOM- 20101029
LR  - 20211020
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 116
IP  - 11
DP  - 2010 Sep 16
TI  - Inhibition of lipid antigen presentation in dendritic cells by HIV-1 Vpu 
      interference with CD1d recycling from endosomal compartments.
PG  - 1876-84
LID - 10.1182/blood-2009-09-243667 [doi]
AB  - Dendritic cells (DCs) play an important role in viral infections both as 
      initiators of immunity and as viral targets. Interaction between DCs and the 
      innate-like CD1d-restricted natural killer T (NKT) cells results in the mutual 
      activation of both cells and the subsequent initiation of cellular immune 
      responses. Here, we show that HIV-1 inhibits the surface expression of CD1d in 
      productively infected DCs and identify this as a novel activity of the HIV-1 vpu 
      gene product. Interestingly, the viral protein U (Vpu) does not enhance 
      constitutive CD1d endocytosis or induce rapid CD1d degradation. Instead, the Vpu 
      protein interacts with CD1d and suppresses its recycling from endosomal 
      compartments to the cell surface by retaining CD1d in early endosomes. This 
      interference with the CD1d antigen presentation pathway strongly inhibits the 
      ability of infected DCs to activate CD1d-restricted NKT cells. Given that the 
      interaction with CD1d-expressing DCs is central to the ability of NKT cells to 
      regulate immunity, these data suggest that interference with the CD1d antigen 
      presentation pathway represents an HIV-1 strategy to evade innate cellular immune 
      responses and imply a role for the innate-like CD1d-restricted NKT cells in the 
      host defense against HIV-1.
FAU - Moll, Markus
AU  - Moll M
AD  - Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, 
      Karolinska University Hospital Huddinge, Stockholm, Sweden. markus.moll@ki.se
FAU - Andersson, Sofia K
AU  - Andersson SK
FAU - Smed-Sorensen, Anna
AU  - Smed-Sorensen A
FAU - Sandberg, Johan K
AU  - Sandberg JK
LA  - eng
GR  - R01 AI052731/AI/NIAID NIH HHS/United States
GR  - R37 AI052731/AI/NIAID NIH HHS/United States
GR  - AI52731/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100608
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD1)
RN  - 0 (Antigens, CD1d)
RN  - 0 (Human Immunodeficiency Virus Proteins)
RN  - 0 (Lipids)
RN  - 0 (Viral Regulatory and Accessory Proteins)
RN  - 0 (vpu protein, Human immunodeficiency virus 1)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD1/metabolism
MH  - Antigens, CD1d/genetics/*immunology/metabolism
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Membrane/immunology/metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/metabolism/virology
MH  - Endocytosis/immunology
MH  - Endosomes/*immunology/metabolism
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - HIV-1/genetics/immunology/metabolism
MH  - Human Immunodeficiency Virus Proteins/genetics/*immunology/metabolism
MH  - Humans
MH  - Lipids/immunology
MH  - Lymphocyte Activation/immunology
MH  - Microscopy, Fluorescence
MH  - Natural Killer T-Cells/immunology/metabolism
MH  - Protein Binding
MH  - Viral Regulatory and Accessory Proteins/genetics/*immunology/metabolism
PMC - PMC3173986
EDAT- 2010/06/10 06:00
MHDA- 2010/10/30 06:00
PMCR- 2011/09/16
CRDT- 2010/06/10 06:00
PHST- 2010/06/10 06:00 [entrez]
PHST- 2010/06/10 06:00 [pubmed]
PHST- 2010/10/30 06:00 [medline]
PHST- 2011/09/16 00:00 [pmc-release]
AID - S0006-4971(20)32990-6 [pii]
AID - 2009/243667 [pii]
AID - 10.1182/blood-2009-09-243667 [doi]
PST - ppublish
SO  - Blood. 2010 Sep 16;116(11):1876-84. doi: 10.1182/blood-2009-09-243667. Epub 2010 
      Jun 8.