PMID- 20532892
OWN - NLM
STAT- MEDLINE
DCOM- 20110203
LR  - 20220408
IS  - 1432-136X (Electronic)
IS  - 0174-1578 (Linking)
VI  - 180
IP  - 8
DP  - 2010 Nov
TI  - Redox homeostasis and respiratory metabolism in camels (Camelus dromedaries): 
      comparisons with domestic goats and laboratory rats and mice.
PG  - 1121-32
LID - 10.1007/s00360-010-0482-x [doi]
AB  - We have previously reported the occurrence of multiple forms of drug-metabolizing 
      enzymes in camel tissues. Here, we investigate glutathione (GSH)-dependent redox 
      homeostasis, reactive oxygen species (ROS) production and mitochondrial 
      respiratory functions in camel tissues and compare them with imported domestic 
      goats and laboratory rats and mice. Cytochrome P450 2E1 (CYP 2E1) and 
      GSH-metabolizing enzymes were differentially expressed in the liver and kidney of 
      these animals. Camel liver has significantly lower GSH pool than that in goats, 
      rats and mice. Mitochondria isolated from the tissues of these animals showed a 
      comparable ability to metabolize specific substrates for respiratory enzyme 
      complexes I, II/III and IV. These complexes were metabolically more active in the 
      kidney than in the liver of all the species. Furthermore, the activity of complex 
      IV in camel tissues was significantly lower than in other species. On the other 
      hand, complex II/III activity in camel kidney was higher compared to the other 
      species. In addition, as expected, we observed that inhibitors of these enzyme 
      complexes augment the production of mitochondrial ROS in camel and goat tissues. 
      These results help to better understand the metabolic ability and adaptation in 
      desert camels in comparison with domestic goats and laboratory rats and mice 
      since they are exposed to different environmental and dietary conditions. Our 
      study may also have implications in the pharmacology and toxicology of drugs and 
      pollutants in these species.
FAU - Al-Otaiba, Amna
AU  - Al-Otaiba A
AD  - Department of Biochemistry, Faculty of Medicine and Health Sciences, UAE 
      University, PO Box 17666, Al Ain, United Arab Emirates.
FAU - John, Annie
AU  - John A
FAU - Al-Belooshi, Thekra
AU  - Al-Belooshi T
FAU - Raza, Haider
AU  - Raza H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100609
PL  - Germany
TA  - J Comp Physiol B
JT  - Journal of comparative physiology. B, Biochemical, systemic, and environmental 
      physiology
JID - 8413200
RN  - 0 (Electron Transport Chain Complex Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.14.13.- (Cytochrome P-450 CYP2E1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Camelus/metabolism/*physiology
MH  - Cytochrome P-450 CYP2E1/metabolism
MH  - Electron Transport Chain Complex Proteins/metabolism
MH  - Glutathione/*metabolism
MH  - Glutathione Transferase/metabolism
MH  - Goats/metabolism/physiology
MH  - Homeostasis
MH  - Inactivation, Metabolic
MH  - Kidney/metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitochondria/metabolism
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Species Specificity
MH  - Tissue Distribution
EDAT- 2010/06/10 06:00
MHDA- 2011/02/04 06:00
CRDT- 2010/06/10 06:00
PHST- 2010/01/28 00:00 [received]
PHST- 2010/05/20 00:00 [accepted]
PHST- 2010/05/16 00:00 [revised]
PHST- 2010/06/10 06:00 [entrez]
PHST- 2010/06/10 06:00 [pubmed]
PHST- 2011/02/04 06:00 [medline]
AID - 10.1007/s00360-010-0482-x [doi]
PST - ppublish
SO  - J Comp Physiol B. 2010 Nov;180(8):1121-32. doi: 10.1007/s00360-010-0482-x. Epub 
      2010 Jun 9.