PMID- 20533298
OWN - NLM
STAT- MEDLINE
DCOM- 20110125
LR  - 20181201
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 111
IP  - 2
DP  - 2010 Oct 1
TI  - Mechanism of TNF-alpha-induced migration and hepatocyte growth factor production in 
      human mesenchymal stem cells.
PG  - 469-75
LID - 10.1002/jcb.22729 [doi]
AB  - Accumulating evidence suggests that mesenchymal stem cells (MSCs) may decrease 
      destructive inflammation and reduce tissue loss. Tumor necrosis factor-alpha (TNF-alpha) 
      plays a central role in induction of proinflammatory signaling and paradoxically 
      activates intracellular anti-inflammatory survival pathways. In this study, we 
      investigated whether TNF-alpha could induce a chemotactic effect on human MSCs and 
      stimulate their production of anti-inflammatory factors in vitro, as well as 
      determined mechanisms that mediated this effect. Migration assays demonstrated 
      that TNF-alpha had a chemotactic effect on MSCs. TNF-alpha increased both hepatocyte 
      growth factor (HGF) mRNA expression in MSCs and HGF secretion in conditioned 
      medium. These effects were dependent on the p38 MAPK and PI3K/Akt, but not JNK 
      and ERK signaling pathways. Furthermore, these effects were inhibited by a 
      specific neutralizing antibody to TNF receptor II, but not TNF receptor I. We 
      conclude that TNF-alpha can enhance human MSCs migration and stimulate their 
      production of HGF. These effects are mediated via a specific TNF receptor and 
      signaling pathways.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Zhang, Aibin
AU  - Zhang A
AD  - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated 
      Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
FAU - Wang, Yan
AU  - Wang Y
FAU - Ye, Zhou
AU  - Ye Z
FAU - Xie, Haiyang
AU  - Xie H
FAU - Zhou, Lin
AU  - Zhou L
FAU - Zheng, Shusen
AU  - Zheng S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - Hepatocyte Growth Factor/*biosynthesis
MH  - Humans
MH  - Mesenchymal Stem Cells
MH  - RNA, Messenger/analysis
MH  - Receptors, Tumor Necrosis Factor
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2010/06/10 06:00
MHDA- 2011/01/28 06:00
CRDT- 2010/06/10 06:00
PHST- 2010/06/10 06:00 [entrez]
PHST- 2010/06/10 06:00 [pubmed]
PHST- 2011/01/28 06:00 [medline]
AID - 10.1002/jcb.22729 [doi]
PST - ppublish
SO  - J Cell Biochem. 2010 Oct 1;111(2):469-75. doi: 10.1002/jcb.22729.