PMID- 20533306
OWN - NLM
STAT- MEDLINE
DCOM- 20101012
LR  - 20100915
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 225
IP  - 3
DP  - 2010 Nov
TI  - Carbonic anhydrase II regulates differentiation of ameloblasts via intracellular 
      pH-dependent JNK signaling pathway.
PG  - 709-19
LID - 10.1002/jcp.22267 [doi]
AB  - Differentiation of ameloblasts from undifferentiated epithelial cells is 
      controlled by diverse growth factors, as well as interactions between epithelium 
      and mesenchyme. However, there is a considerable lack of knowledge regarding the 
      precise mechanisms that control ameloblast differentiation and enamel 
      biomineralization. We found that the expression level of carbonic anhydrase II 
      (CAII) is strongly up-regulated in parallel with differentiation of enamel 
      epithelium tissues, while the enzyme activity of CA was also increased along with 
      differentiation in ameloblast primary cultures. The expression level of 
      amelogenin, a marker of secretory-stage ameloblasts, was enhanced by 
      ethoxzolamide (EZA), a CA inhibitor, as well as CAII antisense (CAIIAS), whereas 
      the expression of enamel matrix serine proteinase-1 (EMSP-1), a marker for 
      maturation-stage ameloblasts, was suppressed by both. These agents also promoted 
      ameloblast proliferation. In addition, inhibition of ameloblast differentiation 
      by EZA and CAIIAS was confirmed using tooth germ organ cultures. Furthermore, EZA 
      and CAIIAS elevated intracellular pH in ameloblasts, while experimental decreases 
      in intracellular pH abolished the effect of CAIIAS on ameloblasts and triggered 
      the activation of c-Jun N-terminal kinase (JNK). SP600125, a JNK inhibitor, 
      abrogated the response of ameloblasts to an experimental decrease in 
      intracellular pH, while the inhibition of JNK also impaired ameloblast 
      differentiation. These results suggest a novel role for CAII during amelogenesis, 
      that is, controlling the differentiation of ameloblasts. Regulation of 
      intracellular pH, followed by activation of the JNK signaling pathway, may be 
      responsible for the effects of CAII on ameloblasts.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Wang, Xiaogu
AU  - Wang X
AD  - Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
FAU - Suzawa, Tetsuo
AU  - Suzawa T
FAU - Ohtsuka, Hirotada
AU  - Ohtsuka H
FAU - Zhao, Baohong
AU  - Zhao B
FAU - Miyamoto, Yoichi
AU  - Miyamoto Y
FAU - Miyauchi, Tomohiko
AU  - Miyauchi T
FAU - Nishimura, Riko
AU  - Nishimura R
FAU - Inoue, Tomio
AU  - Inoue T
FAU - Nakamura, Masanori
AU  - Nakamura M
FAU - Baba, Kazuyoshi
AU  - Baba K
FAU - Kamijo, Ryutaro
AU  - Kamijo R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Amelogenin)
RN  - 0 (Carbonic Anhydrase Inhibitors)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 3.4.21.- (Kallikreins)
RN  - EC 3.4.21.- (kallikrein 4)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Ameloblasts/drug effects/*enzymology
MH  - Amelogenin/metabolism
MH  - Animals
MH  - Carbonic Anhydrase II/antagonists & inhibitors/genetics/*metabolism
MH  - Carbonic Anhydrase Inhibitors/pharmacology
MH  - *Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Gene Expression Regulation, Enzymologic
MH  - Hydrogen-Ion Concentration
MH  - JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Kallikreins/metabolism
MH  - Mice
MH  - Oligonucleotides, Antisense/metabolism
MH  - Organ Culture Techniques
MH  - Protein Kinase Inhibitors/pharmacology
MH  - RNA, Messenger/metabolism
MH  - *Signal Transduction/drug effects
MH  - Time Factors
MH  - Tooth Germ/cytology/drug effects/*enzymology
EDAT- 2010/06/10 06:00
MHDA- 2010/10/13 06:00
CRDT- 2010/06/10 06:00
PHST- 2010/06/10 06:00 [entrez]
PHST- 2010/06/10 06:00 [pubmed]
PHST- 2010/10/13 06:00 [medline]
AID - 10.1002/jcp.22267 [doi]
PST - ppublish
SO  - J Cell Physiol. 2010 Nov;225(3):709-19. doi: 10.1002/jcp.22267.