PMID- 20533943
OWN - NLM
STAT- MEDLINE
DCOM- 20101008
LR  - 20171116
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 73
IP  - 2
DP  - 2010 Aug
TI  - Quantification of catechol dioxygenase gene expression in soil during degradation 
      of 2,4-dichlorophenol.
PG  - 363-9
LID - 10.1111/j.1574-6941.2010.00906.x [doi]
AB  - The tfdC and C23O genes encode two catechol dioxygenases that catalyse ortho and 
      meta cleavage of a key metabolite (chlorocatechol) of 2,4-dichlorophenol 
      (2,4-DCP) metabolism, respectively. Primers were designed and a real-time PCR 
      assay was developed to assess the abundance and expression of both tfdC and C23O 
      genes in a soil amended with 2,4-DCP over a 21-day period. tfdC, the gene 
      encoding the ortho cleaving dioxygenase, was significantly more abundant than the 
      meta cleaving dioxygenase gene (C23O) throughout the experiment. The highest 
      levels of tfdC were observed 2 days after amendment of soil with 2,4-DCP, at 
      which stage the rate of 2,4-DCP degradation was at its maximum. In contrast, C230 
      copy numbers declined initially and peaked when degradation had slowed 
      considerably. mRNA of the two chlorocatechol dioxygenase genes was not detected 
      on day 0, but both genes were expressed after this time point. tfdC was expressed 
      at a significantly higher level than C23O in 2,4-DCP-amended soil throughout the 
      course of the microcosm, indicating the dominance of the ortho metabolic pathway. 
      Phylogenetic analysis revealed a wide diversity of chlorocatechol dioxygenase 
      genes in the 2,4-DCP-exposed soil examined.
FAU - Lillis, Lorraine
AU  - Lillis L
AD  - School of Biology and Environmental Science, Ardmore House, University College 
      Dublin, Dublin, Ireland.
FAU - Clipson, Nicholas
AU  - Clipson N
FAU - Doyle, Evelyn
AU  - Doyle E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100510
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Bacterial Proteins)
RN  - 0 (Catechols)
RN  - 0 (Chlorophenols)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Soil)
RN  - EC 1.13.11.- (Dioxygenases)
RN  - EC 1.13.11.- (chlorocatechol 1,2-dioxygenase)
RN  - EC 1.13.11.2 (Catechol 2,3-Dioxygenase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Bacteria/enzymology/genetics
MH  - Bacterial Proteins/genetics/isolation & purification/metabolism
MH  - Biodegradation, Environmental
MH  - Catechol 2,3-Dioxygenase/genetics/*isolation & purification/metabolism
MH  - Catechols/metabolism
MH  - Chlorophenols/*metabolism
MH  - DNA, Bacterial/isolation & purification
MH  - Dioxygenases/genetics/*isolation & purification/metabolism
MH  - Gene Library
MH  - Genes, Bacterial
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - Soil/*analysis
MH  - *Soil Microbiology
EDAT- 2010/06/11 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/06/11 06:00
PHST- 2010/06/11 06:00 [entrez]
PHST- 2010/06/11 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
AID - FEM906 [pii]
AID - 10.1111/j.1574-6941.2010.00906.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2010 Aug;73(2):363-9. doi: 10.1111/j.1574-6941.2010.00906.x. 
      Epub 2010 May 10.