PMID- 20534450
OWN - NLM
STAT- MEDLINE
DCOM- 20100714
LR  - 20231213
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 107
IP  - 24
DP  - 2010 Jun 15
TI  - Expansion of human NK-22 cells with IL-7, IL-2, and IL-1beta reveals intrinsic 
      functional plasticity.
PG  - 10961-6
LID - 10.1073/pnas.1005641107 [doi]
AB  - Natural killer-22 (NK-22) cells are a human NK cell subset situated in 
      mucosal-associated lymphoid tissues that specialize in IL-22 secretion in 
      response to IL-23. Here we investigated the cytokine requirements for NK-22 cell 
      expansion. IL-7 maintained the survival of NK-22 cells and IL-22 production in 
      response to IL-23 but was insufficient to induce robust expansion. Proliferation 
      of NK-22 cells was increased markedly by adding either IL-1beta or IL-2 to IL-7 
      and was even stronger in the presence of IL-1beta plus IL-2. In contrast to IL-7, 
      continuous culture in IL-1beta and IL-2 modified NK-22 cytokine profiles. 
      IL-1beta promoted constitutive IL-22 secretion rather than acute IL-22 production 
      in response to IL-23 and induced IL-17 in some cells. IL-2 reduced secretion of 
      IL-22 and IL-17, increasing production of IFN-gamma and leukemia inhibitory 
      factor. Functional deviation toward IFN-gamma production also was induced by 
      continuous culture in IL-23. These results demonstrate the functional plasticity 
      of NK-22 cells, which may allow flexible responses to different pathogens. 
      Finally, we found that NK-22 cells released the B-cell survival factor, B-cell 
      activating factor belonging to the TNF family (BAFF), suggesting a potential role 
      of NK-22 cells in promoting B-cell-mediated mucosal immunity.
FAU - Cella, Marina
AU  - Cella M
AD  - Department of Pathology and Immunology, Washington University School of Medicine, 
      St Louis, MO 63110, USA.
FAU - Otero, Karel
AU  - Otero K
FAU - Colonna, Marco
AU  - Colonna M
LA  - eng
GR  - P30 DK056341/DK/NIDDK NIH HHS/United States
GR  - P30 DK056341-10/DK/NIDDK NIH HHS/United States
GR  - A1067854/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100601
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (B-Cell Activating Factor)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (IL2 protein, human)
RN  - 0 (IL23A protein, human)
RN  - 0 (IL7 protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukin-23 Subunit p19)
RN  - 0 (Interleukin-7)
RN  - 0 (Interleukins)
RN  - 0 (LIF protein, human)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TNFSF13B protein, human)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - B-Cell Activating Factor/biosynthesis
MH  - Base Sequence
MH  - Cell Proliferation/drug effects
MH  - Cytokines/biosynthesis/genetics
MH  - DNA Primers/genetics
MH  - Humans
MH  - In Vitro Techniques
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-1beta/*pharmacology
MH  - Interleukin-2/*pharmacology
MH  - Interleukin-23 Subunit p19/pharmacology
MH  - Interleukin-7/*pharmacology
MH  - Interleukins/*biosynthesis
MH  - Killer Cells, Natural/*classification/cytology/drug effects/*immunology
MH  - Leukemia Inhibitory Factor/biosynthesis
MH  - Lymphocyte Subsets/*classification/cytology/drug effects/*immunology
MH  - Palatine Tonsil/cytology/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Interleukin-22
PMC - PMC2890739
COIS- The authors declare no conflict of interest.
EDAT- 2010/06/11 06:00
MHDA- 2010/07/16 06:00
PMCR- 2010/06/01
CRDT- 2010/06/11 06:00
PHST- 2010/06/11 06:00 [entrez]
PHST- 2010/06/11 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
PHST- 2010/06/01 00:00 [pmc-release]
AID - 1005641107 [pii]
AID - 201005641 [pii]
AID - 10.1073/pnas.1005641107 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10961-6. doi: 
      10.1073/pnas.1005641107. Epub 2010 Jun 1.