PMID- 20535496
OWN - NLM
STAT- MEDLINE
DCOM- 20110519
LR  - 20211020
IS  - 1432-1335 (Electronic)
IS  - 0171-5216 (Linking)
VI  - 137
IP  - 4
DP  - 2011 Apr
TI  - Helicase-like transcription factor confers radiation resistance in cervical 
      cancer through enhancing the DNA damage repair capacity.
PG  - 629-37
LID - 10.1007/s00432-010-0925-5 [doi]
AB  - Helicase-like transcription factor (HLTF) is a member of the SWI/SNF (mating type 
      switching/sucrose non-fermenting) family of ATPases/helicases and also has a 
      RING-finger motif characteristic of ubiquitin ligase proteins. These features 
      have led to suggestions that HLTF functions like yeast Rad5, which promotes 
      replication through DNA lesions via a post-replication repair pathway. However, 
      the function of HLTF in higher eukaryotes is still unknown. Herein, we found the 
      overexpression of HLTF in radiation recurrent human uterine cervical carcinoma 
      tissues when compared to disease free survived patients tissues. In this study, 
      we used RNA interference techniques to investigate the potential function of HLTF 
      in cervical cancer cell line HeLa and found that the cell proliferation was 
      reduced by knockdown (KD) of HLTF. A host-cell reactivation assay showed that the 
      capacity for repair to DNA damage induced by X-ray irradiation was reduced in 
      HLTF KD cells. X-rays also increased apoptosis in HLTF KD cells. These results 
      suggest that HLTF is involved in DNA repair and apoptosis in cancer cells, which 
      might represent a target for gene therapies of human cancer.
FAU - Cho, SungHwan
AU  - Cho S
AD  - Department of Radiation Oncology, Chungbuk National University College of 
      Medicine, Cheongju 361-763, Republic of Korea.
FAU - Cinghu, Senthilkumar
AU  - Cinghu S
FAU - Yu, Jae-Ran
AU  - Yu JR
FAU - Park, Woo-Yoon
AU  - Park WY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100610
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HLTF protein, human)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Apoptosis
MH  - Cell Proliferation
MH  - DNA Damage
MH  - *DNA Repair
MH  - DNA-Binding Proteins/analysis/*physiology
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - RNA, Small Interfering/genetics
MH  - *Radiation Tolerance
MH  - Transcription Factors/analysis/*physiology
MH  - Uterine Cervical Neoplasms/pathology/*radiotherapy
EDAT- 2010/06/11 06:00
MHDA- 2011/05/20 06:00
CRDT- 2010/06/11 06:00
PHST- 2010/03/21 00:00 [received]
PHST- 2010/05/22 00:00 [accepted]
PHST- 2010/06/11 06:00 [entrez]
PHST- 2010/06/11 06:00 [pubmed]
PHST- 2011/05/20 06:00 [medline]
AID - 10.1007/s00432-010-0925-5 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 2011 Apr;137(4):629-37. doi: 10.1007/s00432-010-0925-5. 
      Epub 2010 Jun 10.