PMID- 20535563
OWN - NLM
STAT- MEDLINE
DCOM- 20110526
LR  - 20220309
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 38
IP  - 2
DP  - 2011 Feb
TI  - Microarray analysis of cultured rat hippocampal neurons treated with brain 
      derived neurotrophic factor.
PG  - 983-90
LID - 10.1007/s11033-010-0193-0 [doi]
AB  - Brain derived neurotrophic factor (BDNF) has been shown to exert multiple actions 
      on neurons. It plays a role in neuronal growth and maintenance and use-dependent 
      plasticity, such as long-term potentiation and learning. This neurotrophin is 
      believed to regulate neuronal plasticity by modifying neuronal excitability and 
      morphology. There is experimental evidence for both an acute and a long-term 
      effect of BDNF on synaptic transmission and structure but the molecular 
      mechanisms underlying these events have not been completely clarified. In order 
      to study the BDNF-induced molecular changes, the set of genes modulated in 
      cultured hippocampal neurons by BDNF treatment was investigated after subchronic 
      treatment with the neurotrophin. Microarray analysis performed with these cells, 
      revealed increased expression of mRNA encoding the neuropeptides neuropeptide Y 
      and somatostatin, and of the secreted peptide VGF (non acronymic), all of which 
      participate in neurotransmission. In addition, the expression of genes 
      apolipoprotein E (ApoE), delta-6 fatty acid desaturase (Fads2) and matrix 
      metalloproteinase 14 (Mmp14), which play a role in neuronal remodelling, was also 
      enhanced. More studies are needed to investigate and confirm the role of these 
      genes in synaptic plasticity, but the results reported in this paper show that 
      microarray analysis of hippocampal cultures can be used to expand our current 
      knowledge of the molecular events triggered by BDNF in the hippocampus.
FAU - Cazzin, Chiara
AU  - Cazzin C
AD  - Neuroscience Centre of Excellence for Drug Discovery, GlaxoSmithKline, Medicines 
      Research Center, via Fleming, 4, 37135, Verona, Italy. chiara.cazzin@gsk.com
FAU - Mion, Silvia
AU  - Mion S
FAU - Caldara, Fabrizio
AU  - Caldara F
FAU - Rimland, Joseph M
AU  - Rimland JM
FAU - Domenici, Enrico
AU  - Domenici E
LA  - eng
PT  - Journal Article
DEP - 20100610
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Apolipoproteins E)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuropeptides)
RN  - EC 1.14.19.1 (Stearoyl-CoA Desaturase)
RN  - EC 1.14.99.- (delta 6-desaturase, rat)
RN  - EC 3.4.24.80 (Matrix Metalloproteinase 14)
SB  - IM
MH  - Animals
MH  - Apolipoproteins E/biosynthesis
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Hippocampus/embryology/*metabolism
MH  - Matrix Metalloproteinase 14/biosynthesis
MH  - Models, Biological
MH  - Neuronal Plasticity
MH  - Neuropeptides/chemistry
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stearoyl-CoA Desaturase/biosynthesis
MH  - Synaptic Transmission
EDAT- 2010/06/11 06:00
MHDA- 2011/05/27 06:00
CRDT- 2010/06/11 06:00
PHST- 2010/01/11 00:00 [received]
PHST- 2010/05/21 00:00 [accepted]
PHST- 2010/06/11 06:00 [entrez]
PHST- 2010/06/11 06:00 [pubmed]
PHST- 2011/05/27 06:00 [medline]
AID - 10.1007/s11033-010-0193-0 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2011 Feb;38(2):983-90. doi: 10.1007/s11033-010-0193-0. Epub 2010 
      Jun 10.