PMID- 20536357
OWN - NLM
STAT- MEDLINE
DCOM- 20110214
LR  - 20181201
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 16
IP  - 11
DP  - 2010 Nov
TI  - Osteogenic potential of human mesenchymal stem cells and human embryonic stem 
      cell-derived mesodermal progenitors: a tissue engineering perspective.
PG  - 3413-26
LID - 10.1089/ten.TEA.2010.0052 [doi]
AB  - INTRODUCTION: Human mesenchymal stem cells (hMSCs) are promising candidates for 
      bone engineering and regeneration with a considerable number of experimental 
      successes reported over the last years. However, hMSCs show several limitations 
      for tissue engineering applications, which can be overcome by using human 
      embryonic stem cell-derived mesodermal progenitors (hES-MPs). The aim of this 
      study was to investigate and compare the osteogenic differentiation potential of 
      hMSCs and hES-MPs. MATERIALS AND METHODS: The osteogenic differentiation and 
      mineralization behavior of both cell types were evaluated at passage 5, 10, 15, 
      and 20. Expression of COL1A1, RUNX2, OPN, and OC was evaluated by reverse 
      transcription (RT)-polymerase chain reaction, whereas mineralization was examined 
      by photospectrometry, von Kossa staining, and time-of-flight secondary ion mass 
      spectrometry. The immunoprofile of both cell types was investigated by flow 
      cytometry. RESULTS: We demonstrated that, under proper stimulation, hES-MPs 
      undergo osteogenic differentiation and exhibit significantly increased 
      mineralization ability compared to hMSCs after protracted expansion. hES-MPs were 
      also found to express lower amount of human leukocyte antigens class II proteins. 
      CONCLUSIONS: The high osteogenic ability of hES-MPs, together with low expression 
      of human leukocyte antigens class II, makes these cells an attractive alternative 
      for bulk production of cells for bone engineering applications.
FAU - de Peppo, Giuseppe Maria
AU  - de Peppo GM
AD  - Department of Biomaterials, Sahlgrenska Academy at University of Gothenburg, 
      Gothenburg, Sweden. giuseppe.de.peppo@biomaterials.gu.se
FAU - Sjovall, Peter
AU  - Sjovall P
FAU - Lenneras, Maria
AU  - Lenneras M
FAU - Strehl, Raimund
AU  - Strehl R
FAU - Hyllner, Johan
AU  - Hyllner J
FAU - Thomsen, Peter
AU  - Thomsen P
FAU - Karlsson, Camilla
AU  - Karlsson C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100802
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (HLA Antigens)
RN  - 0 (Phosphates)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adipogenesis/genetics
MH  - Adolescent
MH  - Adult
MH  - Alkaline Phosphatase/metabolism
MH  - Calcification, Physiologic/physiology
MH  - Calcium/metabolism
MH  - Cell Separation
MH  - Embryonic Stem Cells/*cytology/enzymology
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - HLA Antigens/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/enzymology
MH  - Mesoderm/*cytology/enzymology
MH  - *Osteogenesis/genetics
MH  - Phosphates/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spectrometry, Mass, Secondary Ion
MH  - Staining and Labeling
MH  - Tissue Donors
MH  - *Tissue Engineering
EDAT- 2010/06/12 06:00
MHDA- 2011/02/15 06:00
CRDT- 2010/06/12 06:00
PHST- 2010/06/12 06:00 [entrez]
PHST- 2010/06/12 06:00 [pubmed]
PHST- 2011/02/15 06:00 [medline]
AID - 10.1089/ten.TEA.2010.0052 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2010 Nov;16(11):3413-26. doi: 10.1089/ten.TEA.2010.0052. Epub 
      2010 Aug 2.