PMID- 20536548
OWN - NLM
STAT- MEDLINE
DCOM- 20100902
LR  - 20220409
IS  - 1601-5223 (Electronic)
IS  - 0018-0661 (Linking)
VI  - 147
IP  - 2
DP  - 2010 Apr
TI  - Is there a genetic variation association in the IL-10 and TNF alpha promoter gene 
      with gestational diabetes mellitus?
PG  - 94-102
LID - 10.1111/j.1601-5223.2009.02134.x [doi]
AB  - Gestational diabetes mellitus (GDM), defined as carbohydrate intolerance 
      diagnosed for the first time during pregnancy, affects both maternal and fetal 
      health. Possession of a specific genetic polymorphism can be a predisposing 
      factor for susceptibility to some diseases. The aim of this study was to 
      investigate the association between single nucleotide polymorphisms (SNP) in the 
      promoter gene of interleukin-10 (IL-10) as well as tumor necrosis factor-alpha 
      (TNF alpha) with the development of GDM. Two hundred and twelve consecutive 
      series of eligible normal pregnant women (controls) and gestational diabetes 
      mellitus women were selected based on the study's inclusion and exclusion 
      criteria. DNA was extracted from blood and genotyped for IL-10 at three positions 
      and TNF alpha for gene polymorphism using the polymerase chain 
      reaction-restriction fragment length polymorphism (PCR-RFLP) method. Plasma 
      levels of IL-10 and TNF alpha at different gestational periods as well as 
      postpartum were quantified using enzyme linked immunosorbent assay (ELISA). The 
      results of the study showed that the difference in the frequency of SNP at 
      position -597 in the promoter of the human IL-10 gene between the control and GDM 
      groups was statistically significant (p < 0.05). In contrast, there was no 
      significant difference in the frequency of SNP at the other two sites in the 
      promoter region of the human IL-10 gene (-824 and -1082) as well as position -308 
      in the promoter of the human TNF-alpha (p > 0.05). In addition, there was no 
      significant difference between the two groups in terms of plasma levels of IL-10 
      as well as TNF alpha in different stages of pregnancy. SNP at position -597 was 
      significantly associated with the development of GDM and shows potential for use 
      as a predictive marker for GDM.
FAU - Montazeri, Shabnam
AU  - Montazeri S
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine and Health, 
      International Medical University (IMU), Bukit Jalil, Kuala Lumpur, Malaysia. 
      shabnam_montazeri@yahoo.com
FAU - Nalliah, Sivalingam
AU  - Nalliah S
FAU - Radhakrishnan, Ammu Kutty
AU  - Radhakrishnan AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hereditas
JT  - Hereditas
JID - 0374654
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Diabetes, Gestational/*genetics
MH  - Female
MH  - *Genetic Variation
MH  - Humans
MH  - Interleukin-10/*genetics
MH  - Middle Aged
MH  - Pregnancy
MH  - *Promoter Regions, Genetic
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2010/06/12 06:00
MHDA- 2010/09/03 06:00
CRDT- 2010/06/12 06:00
PHST- 2010/06/12 06:00 [entrez]
PHST- 2010/06/12 06:00 [pubmed]
PHST- 2010/09/03 06:00 [medline]
AID - HRD2134 [pii]
AID - 10.1111/j.1601-5223.2009.02134.x [doi]
PST - ppublish
SO  - Hereditas. 2010 Apr;147(2):94-102. doi: 10.1111/j.1601-5223.2009.02134.x.