PMID- 20536931
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20231213
IS  - 1749-6632 (Electronic)
IS  - 0077-8923 (Linking)
VI  - 1198
DP  - 2010 Jun
TI  - Role of NKCC1 and KCC2 in the development of chronic neuropathic pain following 
      spinal cord injury.
PG  - 168-72
LID - 10.1111/j.1749-6632.2010.05462.x [doi]
AB  - Neuropathic pain is a common problem following spinal cord injury (SCI). 
      Effective analgesic therapy has been hampered by the lack of knowledge about the 
      mechanisms underlying post-SCI neuropathic pain. Current evidence suggests 
      GABAergic spinal nociceptive processing is a critical functional node in this 
      complex phenotype, representing a potential target for therapeutic intervention. 
      Normal GABA neurotransmission is dependent on precise regulation of the level of 
      intracellular chloride, which is determined by the coordinated activities of two 
      cation/chloride cotransporters (CCCs) in the SLC12 family: the inwardly directed 
      Na(+)-K(+)-Cl(-) cotransporter isoform 1 (NKCC1) and outwardly directed 
      K(+)-Cl(-) cotransporter isoform 2 (KCC2). Inhibition of NKCC1 with its potent 
      antagonist bumetanide reduces pain behavior in rats following SCI. Moreover, the 
      injured spinal cord tissues exhibit a significant transient upregulation of NKCC1 
      protein and a concurrent downregulation of KCC2 protein. Thus, imbalanced 
      function of NKCC1 and KCC2 may contribute to the induction and maintenance of the 
      chronic neuropathic pain following SCI.
FAU - Hasbargen, Tera
AU  - Hasbargen T
AD  - Department of Neurosurgery, University of Wisconsin School of Medicine and Public 
      Health, Madison, Wisconsin, USA.
FAU - Ahmed, Mostafa M
AU  - Ahmed MM
FAU - Miranpuri, Gurwattan
AU  - Miranpuri G
FAU - Li, Lin
AU  - Li L
FAU - Kahle, Kristopher T
AU  - Kahle KT
FAU - Resnick, Daniel
AU  - Resnick D
FAU - Sun, Dandan
AU  - Sun D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Slc12a2 protein, rat)
RN  - 0 (Sodium Potassium Chloride Symporter Inhibitors)
RN  - 0 (Sodium-Potassium-Chloride Symporters)
RN  - 0 (Solute Carrier Family 12, Member 2)
RN  - 0 (Symporters)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpv1 protein, rat)
RN  - 0Y2S3XUQ5H (Bumetanide)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Bumetanide/pharmacology
MH  - Chronic Disease
MH  - Hyperalgesia/*physiopathology
MH  - Inflammation/physiopathology
MH  - Pain/*physiopathology
MH  - Rats
MH  - Sodium Potassium Chloride Symporter Inhibitors/pharmacology
MH  - Sodium-Potassium-Chloride Symporters/drug effects/genetics/*physiology
MH  - Solute Carrier Family 12, Member 2
MH  - Spinal Cord Injuries/complications/*physiopathology
MH  - Symporters/genetics/*physiology
MH  - TRPV Cation Channels/antagonists & inhibitors/physiology
MH  - gamma-Aminobutyric Acid/physiology
MH  - K Cl- Cotransporters
EDAT- 2010/06/12 06:00
MHDA- 2010/07/08 06:00
CRDT- 2010/06/12 06:00
PHST- 2010/06/12 06:00 [entrez]
PHST- 2010/06/12 06:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
AID - NYAS5462 [pii]
AID - 10.1111/j.1749-6632.2010.05462.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2010 Jun;1198:168-72. doi: 10.1111/j.1749-6632.2010.05462.x.