PMID- 20537332
OWN - NLM
STAT- MEDLINE
DCOM- 20101207
LR  - 20131121
IS  - 1873-2380 (Electronic)
IS  - 0021-9290 (Linking)
VI  - 43
IP  - 12
DP  - 2010 Aug 26
TI  - Biomechanical alterations of dendritic cells by co-culturing with K562 CML cells 
      and their potential role in immune escape.
PG  - 2339-47
LID - 10.1016/j.jbiomech.2010.04.028 [doi]
AB  - Dendritic cells (DCs), which are potent antigen presenting cells (APCs), are 
      utilized to deliver the signals essential for the initiation of immune responses. 
      In this study, we used an interdisciplinary approach to characterize the effect 
      of K562 cells, a human chronic myeloid leukemia (CML) cell line, on the 
      biomechanical characteristics and immune functions of DCs. When co-cultured with 
      K562 cells, the biomechanical and immunological characteristics of immature DCs 
      (imDCs) and mature DCs (mDCs) were severely impaired compared with controls. The 
      changes include increased membrane viscoelasticity, reorganized cytoskeleton 
      (F-actin), suppressed capability of antigen uptake, transendothelium migration, 
      and activation of naive T cells. In exploring the mechanisms of these changes, we 
      identified several genes and proteins by microarray analysis and 2D gel 
      electrophoresis. Changes were found in the cytoskeleton-related genes and 
      proteins (such as cofilin1 and profilin1) and matrix-related genes and proteins 
      (such as TIMP1 and MMP9). These findings provide a molecular basis for the 
      biomechanical and immunological changes of DCs in response to K562 and may help 
      to elucidate the mechanism for tumor immune escape.
CI  - 2010 Elsevier Ltd. All rights reserved.
FAU - Xu, Xiaofeng
AU  - Xu X
AD  - Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical 
      University, Beijing 100730, PR China.
FAU - Zeng, Zhu
AU  - Zeng Z
FAU - Yao, Weijuan
AU  - Yao W
FAU - Wang, Xianwei
AU  - Wang X
FAU - Sun, Dagong
AU  - Sun D
FAU - Ka, Weibo
AU  - Ka W
FAU - Zhang, Yingyu
AU  - Zhang Y
FAU - Wang, Xifu
AU  - Wang X
FAU - Chen, Xiaopeng
AU  - Chen X
FAU - Zha, Yi
AU  - Zha Y
FAU - Sun, Li
AU  - Sun L
FAU - Xie, Lide
AU  - Xie L
FAU - Wen, Zongyao
AU  - Wen Z
FAU - Chien, Shu
AU  - Chien S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomech
JT  - Journal of biomechanics
JID - 0157375
RN  - 0 (Actins)
RN  - 0 (Antigens, CD)
RN  - 0 (CFL1 protein, human)
RN  - 0 (Cofilin 1)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (PFN1 protein, human)
RN  - 0 (Profilins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Actins/metabolism
MH  - Antigens, CD/metabolism
MH  - Apoptosis
MH  - Biomechanical Phenomena
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Coculture Techniques
MH  - Cofilin 1/genetics
MH  - Culture Media, Conditioned
MH  - Dendritic Cells/cytology/*immunology/*physiology
MH  - Elasticity
MH  - Endocytosis
MH  - Humans
MH  - K562 Cells
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*immunology
MH  - Matrix Metalloproteinase 9/genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phenotype
MH  - Profilins/genetics
MH  - Proteomics
MH  - RNA, Messenger/genetics/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics
MH  - *Tumor Escape
MH  - Viscosity
EDAT- 2010/06/12 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/06/12 06:00
PHST- 2010/01/28 00:00 [received]
PHST- 2010/04/22 00:00 [revised]
PHST- 2010/04/22 00:00 [accepted]
PHST- 2010/06/12 06:00 [entrez]
PHST- 2010/06/12 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0021-9290(10)00240-X [pii]
AID - 10.1016/j.jbiomech.2010.04.028 [doi]
PST - ppublish
SO  - J Biomech. 2010 Aug 26;43(12):2339-47. doi: 10.1016/j.jbiomech.2010.04.028.