PMID- 20540623
OWN - NLM
STAT- MEDLINE
DCOM- 20101013
LR  - 20211020
IS  - 1091-7691 (Electronic)
IS  - 0895-8378 (Print)
IS  - 0895-8378 (Linking)
VI  - 22
IP  - 8
DP  - 2010 Jul
TI  - Comparative airway inflammatory response of normal volunteers to ozone and 
      lipopolysaccharide challenge.
PG  - 648-56
LID - 10.3109/08958371003610966 [doi]
AB  - Ozone and lipopolysaccharide (LPS) are environmental pollutants with adverse 
      health effects noted in both healthy and asthmatic individuals. The authors and 
      others have shown that inhalation of ozone and LPS both induce airway 
      neutrophilia. Based on these similarities, the authors tested the hypothesis that 
      common biological factors determine response to these two different agents. 
      Fifteen healthy, nonasthmatic volunteers underwent a 0.4 part per million ozone 
      exposure for 2 h while performing intermittent moderate exercise. These same 
      subjects underwent an inhaled LPS challenge with 20,000 LPS units of Clinical 
      Center Reference LPS, with a minimum of 1 month separating these two challenge 
      sessions. Induced sputum was obtained 24 h before and 4-6 h after each exposure 
      session. Sputum was assessed for total and differential cell counts and 
      expression of cell surface proteins as measured by flow cytometry. Sputum 
      supernatants were assayed for cytokine concentration. Both ozone and LPS 
      challenge augmented sputum neutrophils and subjects' responses were significantly 
      correlated (R = .73) with each other. Ozone had greater overall influence on cell 
      surface proteins by modifying both monocytes (CD14, human leukocyte antigen 
      [HLA]-DR, CD11b) and macrophages (CD11b, HLA-DR) versus LPS where CD14 and HLA-DR 
      were modified only on monocytes. However, LPS significantly increased interleukin 
      (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha, with no significant 
      increases seen after ozone challenge. Ozone and LPS exposure in healthy 
      volunteers induce similar neutrophil responses in the airways; however, 
      downstream activation of innate immune responses differ, suggesting that oxidant 
      versus bacterial air pollutants may be mediated by different mechanisms.
FAU - Hernandez, Michelle L
AU  - Hernandez ML
AD  - Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel 
      Hill, North Carolina 27599-7310, USA. michelle_hernandez@med.unc.edu
FAU - Harris, Bradford
AU  - Harris B
FAU - Lay, John C
AU  - Lay JC
FAU - Bromberg, Philip A
AU  - Bromberg PA
FAU - Diaz-Sanchez, David
AU  - Diaz-Sanchez D
FAU - Devlin, Robert B
AU  - Devlin RB
FAU - Kleeberger, Steven R
AU  - Kleeberger SR
FAU - Alexis, Neil E
AU  - Alexis NE
FAU - Peden, David B
AU  - Peden DB
LA  - eng
GR  - P30 ES010126/ES/NIEHS NIH HHS/United States
GR  - UL1RR025746/RR/NCRR NIH HHS/United States
GR  - R01 ES012706/ES/NIEHS NIH HHS/United States
GR  - KL2 RR025746/RR/NCRR NIH HHS/United States
GR  - KL2RR025746/RR/NCRR NIH HHS/United States
GR  - R01 ES012706-05/ES/NIEHS NIH HHS/United States
GR  - R01ES012706/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
RN  - 0 (Air Pollutants)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interleukins)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 66H7ZZK23N (Ozone)
SB  - IM
MH  - Administration, Inhalation
MH  - Adult
MH  - Air Pollutants/immunology/toxicity
MH  - Female
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Immunity, Innate/drug effects
MH  - Interleukins/metabolism
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Lipopolysaccharides/administration & dosage/immunology/*toxicity
MH  - Macrophages/drug effects/metabolism
MH  - Male
MH  - Monocytes/drug effects/metabolism
MH  - Neutrophils/drug effects/metabolism
MH  - Ozone/administration & dosage/immunology/*toxicity
MH  - Phagocytes/*drug effects/immunology/metabolism
MH  - Respiratory System/*drug effects/*immunology
MH  - Sputum/cytology/immunology/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Young Adult
PMC - PMC3162474
MID - NIHMS317879
EDAT- 2010/06/15 06:00
MHDA- 2010/10/14 06:00
PMCR- 2011/08/26
CRDT- 2010/06/15 06:00
PHST- 2010/06/15 06:00 [entrez]
PHST- 2010/06/15 06:00 [pubmed]
PHST- 2010/10/14 06:00 [medline]
PHST- 2011/08/26 00:00 [pmc-release]
AID - 10.3109/08958371003610966 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2010 Jul;22(8):648-56. doi: 10.3109/08958371003610966.