PMID- 20540739
OWN - NLM
STAT- MEDLINE
DCOM- 20100816
LR  - 20211020
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 10
DP  - 2010 Jun 11
TI  - Centrosome clustering and cyclin D1 gene amplification in double minutes are 
      common events in chromosomal unstable bladder tumors.
PG  - 280
LID - 10.1186/1471-2407-10-280 [doi]
AB  - BACKGROUND: Aneuploidy, centrosome abnormalities and gene amplification are 
      hallmarks of chromosome instability (CIN) in cancer. Yet there are no studies of 
      the in vivo behavior of these phenomena within the same bladder tumor. METHODS: 
      Twenty-one paraffin-embedded bladder tumors were analyzed by conventional 
      comparative genome hybridization and fluorescence in situ hybridization (FISH) 
      with a cyclin D1 gene (CCND1)/centromere 11 dual-color probe. Immunofluorescent 
      staining of alpha, beta and gamma tubulin was also performed. RESULTS: Based on 
      the CIN index, defined as the percentage of cells not displaying the modal number 
      for chromosome 11, tumors were classified as CIN-negative and CIN-positive. 
      Fourteen out of 21 tumors were considered CIN-positive. All T1G3 tumors were 
      included in the CIN-positive group whereas the majority of Ta samples were 
      classified as CIN-negative tumors. Centrosome clustering was observed in six out 
      of 12 CIN-positive tumors analyzed. CCND1 amplification in homogeneously staining 
      regions was present in six out of 14 CIN-positive tumors; three of them also 
      showed amplification of this gene in double minutes. CONCLUSIONS: Complex in vivo 
      behavior of CCND1 amplicon in bladder tumor cells has been demonstrated by 
      accurate FISH analysis on paraffin-embedded tumors. Positive correlation between 
      high heterogeneity, centrosome abnormalities and CCND1 amplification was found in 
      T1G3 bladder carcinomas. This is the first study to provide insights into the 
      coexistence of CCND1 amplification in homogeneously staining regions and double 
      minutes in primary bladder tumors. It is noteworthy that those patients whose 
      tumors showed double minutes had a significantly shorter overall survival rate (p 
      < 0.001).
FAU - Del Rey, Javier
AU  - Del Rey J
AD  - Departament de Biologia Cellular Fisiologia i Immunologia, Institut de 
      Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 08193 
      Bellaterra, Spain.
FAU - Prat, Esther
AU  - Prat E
FAU - Ponsa, Immaculada
AU  - Ponsa I
FAU - Lloreta, Josep
AU  - Lloreta J
FAU - Gelabert, Antoni
AU  - Gelabert A
FAU - Algaba, Ferran
AU  - Algaba F
FAU - Camps, Jordi
AU  - Camps J
FAU - Miro, Rosa
AU  - Miro R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100611
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CCND1 protein, human)
RN  - 0 (Tubulin)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*genetics
MH  - Centrosome/chemistry/*pathology
MH  - *Chromosomal Instability
MH  - *Chromosomes, Human, Pair 11
MH  - Comparative Genomic Hybridization
MH  - Cyclin D1/*genetics
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - *Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kaplan-Meier Estimate
MH  - Male
MH  - Micronuclei, Chromosome-Defective
MH  - Middle Aged
MH  - Mitosis
MH  - Neoplasm Staging
MH  - Paraffin Embedding
MH  - Prognosis
MH  - Time Factors
MH  - Tubulin/analysis
MH  - Urinary Bladder Neoplasms/chemistry/*genetics/mortality/*pathology
PMC - PMC2906479
EDAT- 2010/06/15 06:00
MHDA- 2010/08/17 06:00
PMCR- 2010/06/11
CRDT- 2010/06/15 06:00
PHST- 2009/11/24 00:00 [received]
PHST- 2010/06/11 00:00 [accepted]
PHST- 2010/06/15 06:00 [entrez]
PHST- 2010/06/15 06:00 [pubmed]
PHST- 2010/08/17 06:00 [medline]
PHST- 2010/06/11 00:00 [pmc-release]
AID - 1471-2407-10-280 [pii]
AID - 10.1186/1471-2407-10-280 [doi]
PST - epublish
SO  - BMC Cancer. 2010 Jun 11;10:280. doi: 10.1186/1471-2407-10-280.