PMID- 20541797
OWN - NLM
STAT- MEDLINE
DCOM- 20101020
LR  - 20100712
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 26
DP  - 2010 Sep
TI  - Bioengineering endothelialized neo-corneas using donor-derived corneal 
      endothelial cells and decellularized corneal stroma.
PG  - 6738-45
LID - 10.1016/j.biomaterials.2010.05.020 [doi]
AB  - Corneal transplantation is a common transplant procedure performed to improve 
      visual acuity by replacing the opaque or distorted host tissue by clear healthy 
      donor tissue. However, its clinical utility is limited due to a lack of high 
      quality donor corneas. Bioengineered neo-corneas, created using an expandable 
      population of human donor-derived corneal endothelial cells (HCEC), could address 
      this current shortage. The objectives of this study were to establish HCEC 
      isolation and culture protocols and to investigate the feasibility of 
      bioengineering corneal tissue constructs by seeding the cells on decellularized 
      human corneal stroma. HCECs were removed from the discarded corneas of eye donors 
      by enzymatic digestion. Cells were expanded and evaluated for their expression of 
      Na(+)/K(+)-ATPase and zona occludens-1 (ZO-1). Donor corneal stromas were cut to 
      120-200 microm thickness slices using a microtome and then decellularized. 
      Extracellular matrix components and mechanical properties of the scaffolds were 
      measured after decellularization. To engineer neo-corneas, 130 HCEC/mm(2) were 
      seeded on decellularized human corneal stromas. The resulting constructs were 
      placed in growth medium for 14 days and then analyzed using scanning electron 
      microscopy (SEM), histology, and immunocytochemistry. Seeded cells retain 
      expression of the functional markers Na(+)/K(+)-ATPase and ZO-1 and constructs 
      have biomechanical properties similar to those of normal corneas. These results 
      indicate that construction of neo-corneas, using HCECs derived from discarded 
      donor corneas and decellularized thin-layer corneal stromas, may create a new 
      source of high quality corneal tissue for transplantation.
CI  - Copyright 2010 Elsevier Ltd. All rights reserved.
FAU - Choi, Jin San
AU  - Choi JS
AD  - Wake Forest Institute for Regenerative Medicine, Wake Forest University Health 
      Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
FAU - Williams, James K
AU  - Williams JK
FAU - Greven, Margaret
AU  - Greven M
FAU - Walter, Keith A
AU  - Walter KA
FAU - Laber, Patrick W
AU  - Laber PW
FAU - Khang, Gilson
AU  - Khang G
FAU - Soker, Shay
AU  - Soker S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100611
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
SB  - IM
MH  - Bioengineering/*methods
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Corneal Stroma/*cytology/*transplantation
MH  - Endothelial Cells/cytology/*transplantation
MH  - Endothelium, Corneal/*cytology/*transplantation
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Mechanical Phenomena
MH  - *Tissue Donors
EDAT- 2010/06/15 06:00
MHDA- 2010/10/21 06:00
CRDT- 2010/06/15 06:00
PHST- 2010/03/22 00:00 [received]
PHST- 2010/05/13 00:00 [accepted]
PHST- 2010/06/15 06:00 [entrez]
PHST- 2010/06/15 06:00 [pubmed]
PHST- 2010/10/21 06:00 [medline]
AID - S0142-9612(10)00638-1 [pii]
AID - 10.1016/j.biomaterials.2010.05.020 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Sep;31(26):6738-45. doi: 10.1016/j.biomaterials.2010.05.020. 
      Epub 2010 Jun 11.