PMID- 20542118
OWN - NLM
STAT- MEDLINE
DCOM- 20101129
LR  - 20150831
IS  - 1096-1186 (Electronic)
IS  - 1043-6618 (Linking)
VI  - 62
IP  - 4
DP  - 2010 Oct
TI  - Quercetin attenuates Monocyte Chemoattractant Protein-1 gene expression in 
      glucose primed aortic endothelial cells through NF-kappaB and AP-1.
PG  - 328-36
LID - 10.1016/j.phrs.2010.06.003 [doi]
AB  - Monocyte Chemoattractant Protein-1 (MCP-1) is involved in the diapedesis of blood 
      monocytes into the arterial intima, an early critical event in atherogenesis. 
      Modulating MCP-1 expression can be a key strategy to decrease the risk for 
      atherosclerosis in diabetes. We hypothesized that quercetin, an anti-inflammatory 
      molecule could modulate high glucose concentration (HG) induced MCP-1 expression 
      in aortic endothelial cells in vitro because of its regulatory effects on 
      Activator Protein-1 (AP-1) and Nuclear Factor-kappaB (NF-kappaB). Rat aortic 
      endothelial cells (RAECs) were exposed to HG in the presence or absence of 
      quercetin. Quercetin attenuated HG induced MCP-1 mRNA (42%) and protein synthesis 
      (45%) when estimated using real-time reverse transcriptase-polymerase chain 
      reaction and enzyme-linked immunosorbent assay respectively. Western blot 
      analysis found quercetin to maintain cytosolic p65 protein levels to that seen in 
      control. Quercetin was found to attenuate HG induced increased NF-kappaB and AP-1 
      DNA binding activity in electrophoretic mobility shift assay. Immunofluorescence 
      studies revealed quercetin to prevent HG induced nuclear localization of p65 and 
      c-jun. Quercetin was also found to decrease HG induced activation of NF-kappaB 
      (71%+/-14%), AP-1 (69%+/-24%) and MCP-1 promoter (79%+/-25%) in EA.hy926 cells 
      when analyzed using luciferase reporter assay. We conclude that quercetin 
      attenuates MCP-1 expression in HG treated RAECs, probably by regulating both 
      NF-kappaB and AP-1 pathways. The findings provide new insights into HG induced 
      MCP-1 gene regulation in aortic endothelial cells and the potential of quercetin 
      in abating the risk for atherosclerosis in diabetes.
CI  - Copyright 2010 Elsevier Ltd. All rights reserved.
FAU - Panicker, Sumith Retnamma
AU  - Panicker SR
AD  - Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for 
      Medical Sciences and Technology, Kerala, India.
FAU - Sreenivas, Prethish
AU  - Sreenivas P
FAU - Babu, Mani Sankar
AU  - Babu MS
FAU - Karunagaran, Devarajan
AU  - Karunagaran D
FAU - Kartha, Chandrasekharan Cheranellore
AU  - Kartha CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100611
PL  - Netherlands
TA  - Pharmacol Res
JT  - Pharmacological research
JID - 8907422
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transcription Factor AP-1)
RN  - 9IKM0I5T1E (Quercetin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Aorta/cytology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Endothelial Cells/*drug effects/metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Glucose/metabolism
MH  - Male
MH  - NF-kappa B/*metabolism
MH  - Promoter Regions, Genetic/drug effects
MH  - Protein Transport/drug effects
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Quercetin/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Transcription Factor AP-1/*metabolism
EDAT- 2010/06/15 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/06/15 06:00
PHST- 2010/01/22 00:00 [received]
PHST- 2010/06/07 00:00 [revised]
PHST- 2010/06/07 00:00 [accepted]
PHST- 2010/06/15 06:00 [entrez]
PHST- 2010/06/15 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S1043-6618(10)00126-X [pii]
AID - 10.1016/j.phrs.2010.06.003 [doi]
PST - ppublish
SO  - Pharmacol Res. 2010 Oct;62(4):328-36. doi: 10.1016/j.phrs.2010.06.003. Epub 2010 
      Jun 11.