PMID- 20544709
OWN - NLM
STAT- MEDLINE
DCOM- 20100713
LR  - 20121115
IS  - 1934-662X (Print)
IS  - 1934-662X (Linking)
VI  - 118
IP  - 3
DP  - 2010 Jun 25
TI  - The application of cytogenetics and fluorescence in situ hybridization to 
      fine-needle aspiration in the diagnosis and subclassification of renal neoplasms.
PG  - 137-45
LID - 10.1002/cncy.20077 [doi]
AB  - BACKGROUND: Percutaneous fine-needle aspiration (FNA) cytology is an important 
      diagnostic test for the evaluation and management of selected renal masses. 
      Cytogenetic analysis of cytology specimens can serve as an adjunct for precise 
      classification because certain tumors are associated with specific chromosomal 
      aberrations. This study summarizes our experience with the application of 
      conventional cytogenetics and fluorescence in situ hybridization (FISH) to renal 
      FNA specimens. METHODS: All percutaneous renal FNAs performed during 2005 through 
      2008 were identified from the electronic pathology database. Results of 
      cytogenetic and FISH analyses were correlated with the final diagnoses of the 
      renal FNAs. RESULTS: A total of 303 renal FNAs were performed. During an onsite 
      assessment, a portion of the cytology specimen was allocated for cytogenetic 
      analysis in 74 cases. Karyotypic analysis or FISH was successful in 44 (59%) of 
      these. Characteristic chromosomal abnormalities were observed in 27 cases. In 17 
      cases, a karyotype revealed a combination of trisomies/tetrasomies and in another 
      5 cases, FISH revealed trisomy 7 and 17, both of which are consistent with 
      papillary renal cell carcinoma (RCC). Two cases showed 3p deletions consistent 
      with clear cell RCC. Trisomy 3 was observed in 1 case of clear cell RCC. Monosomy 
      1 and 17 was observed in a case of papillary RCC comprised oncocytic cells. In 1 
      case of primary renal synovial sarcoma, FISH revealed a rearrangement at the SYT 
      locus (18q11.2). CONCLUSIONS: Renal FNA specimens are amenable to analysis by 
      cytogenetics and FISH in the diagnosis and subclassification of renal neoplasms.
CI  - Copyright 2010 American Cancer Society.
FAU - Roh, Michael H
AU  - Roh MH
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor, 
      Michigan 48109-*5054, USA. mikro@med.umich.edu
FAU - Dal Cin, Paola
AU  - Dal Cin P
FAU - Silverman, Stuart G
AU  - Silverman SG
FAU - Cibas, Edmund S
AU  - Cibas ES
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
RN  - 0 (Organophosphates)
RN  - 126-72-7 (tris(2,3-dibromopropyl)phosphate)
SB  - IM
MH  - *Biopsy, Fine-Needle
MH  - Chromosome Aberrations
MH  - *Cytogenetics
MH  - Feasibility Studies
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*diagnosis/pathology
MH  - Organophosphates
EDAT- 2010/06/15 06:00
MHDA- 2010/07/14 06:00
CRDT- 2010/06/15 06:00
PHST- 2010/06/15 06:00 [entrez]
PHST- 2010/06/15 06:00 [pubmed]
PHST- 2010/07/14 06:00 [medline]
AID - 10.1002/cncy.20077 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2010 Jun 25;118(3):137-45. doi: 10.1002/cncy.20077.