PMID- 20544856
OWN - NLM
STAT- MEDLINE
DCOM- 20100916
LR  - 20161125
IS  - 1098-1136 (Electronic)
IS  - 0894-1491 (Linking)
VI  - 58
IP  - 10
DP  - 2010 Aug
TI  - Intermediate filaments attenuate stimulation-dependent mobility of 
      endosomes/lysosomes in astrocytes.
PG  - 1208-19
LID - 10.1002/glia.21000 [doi]
AB  - Intermediate filament (IF) proteins upregulation is a hallmark of astrocyte 
      activation and reactive gliosis, but its pathophysiological implications remain 
      incompletely understood. A recently reported association between IFs and 
      directional mobility of peptidergic vesicles allows us to hypothesize that IFs 
      affect vesicle dynamics and exocytosis-mediated astrocyte communication with 
      neighboring cells. Here, we ask whether the trafficking of recycling vesicles 
      (i.e., those fused to and then retrieved from the plasma membrane) and 
      endosomes/lysosomes depends on IFs. Recycling vesicles were labeled by antibodies 
      against vesicle glutamate transporter 1 (VGLUT1) and atrial natriuretic peptide 
      (ANP), respectively, and by lysotracker, which labels endosomes/lysosomes. 
      Quantitative fluorescence microscopy was used to monitor the mobility of labeled 
      vesicles in astrocytes, derived from either wild-type (WT) mice or mice deficient 
      in glial fibrillary acidic protein and vimentin (GFAP(-/-)Vim(-/-)), the latter 
      lacking astrocyte IFs. Stimulation with ionomycin or ATP enhanced the mobility of 
      VGLUT1-positive vesicles and reduced the mobility of ANP-positive vesicles in WT 
      astrocytes. In GFAP(-/-)Vim(-/-) astrocytes, both vesicle types responded to 
      stimulation, but the relative increase in mobility of VGLUT1-positive vesicles 
      was more prominent compared with nonstimulated cells, whereas the 
      stimulation-dependent attenuation of ANP-positive vesicles mobility was reduced 
      compared with nonstimulated cells. The mobility of endosomes/lysosomes decreased 
      following stimulation in WT astrocytes. However, in GFAP(-/-)Vim(-/-) astrocytes, 
      a small increase in the mobility of endosomes/lysosomes was observed. These 
      findings show that astrocyte IFs differentially affect the stimulation-dependent 
      mobility of vesicles. We propose that upregulation of IFs in pathologic states 
      may alter the function of astrocytes by deregulating vesicle trafficking.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Potokar, Maja
AU  - Potokar M
AD  - Celica Biomedical Center, Ljubljana, Slovenia.
FAU - Stenovec, Matjaz
AU  - Stenovec M
FAU - Gabrijel, Mateja
AU  - Gabrijel M
FAU - Li, Lizhen
AU  - Li L
FAU - Kreft, Marko
AU  - Kreft M
FAU - Grilc, Sonja
AU  - Grilc S
FAU - Pekny, Milos
AU  - Pekny M
FAU - Zorec, Robert
AU  - Zorec R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Central Nervous System Agents)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Slc17a7 protein, mouse)
RN  - 0 (Vesicular Glutamate Transport Protein 1)
RN  - 0 (Vimentin)
RN  - 0 (glial fibrillary astrocytic protein, mouse)
RN  - 56092-81-0 (Ionomycin)
RN  - 85637-73-6 (Atrial Natriuretic Factor)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Astrocytes/drug effects/*physiology
MH  - Atrial Natriuretic Factor/metabolism
MH  - Biological Transport, Active/drug effects/physiology
MH  - Cells, Cultured
MH  - Central Nervous System Agents/pharmacology
MH  - Cerebral Cortex/drug effects/physiology
MH  - Endosomes/drug effects/*physiology
MH  - Glial Fibrillary Acidic Protein
MH  - Intermediate Filaments/drug effects/*physiology
MH  - Ionomycin/pharmacology
MH  - Lysosomes/drug effects/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Motion
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Vesicular Glutamate Transport Protein 1/metabolism
MH  - Vimentin/genetics/metabolism
EDAT- 2010/06/15 06:00
MHDA- 2010/09/18 06:00
CRDT- 2010/06/15 06:00
PHST- 2010/06/15 06:00 [entrez]
PHST- 2010/06/15 06:00 [pubmed]
PHST- 2010/09/18 06:00 [medline]
AID - 10.1002/glia.21000 [doi]
PST - ppublish
SO  - Glia. 2010 Aug;58(10):1208-19. doi: 10.1002/glia.21000.