PMID- 20545751
OWN - NLM
STAT- MEDLINE
DCOM- 20110131
LR  - 20101029
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 12
IP  - 10
DP  - 2010 Oct
TI  - Inoculum effects on community composition and nitritation performance of 
      autotrophic nitrifying biofilm reactors with counter-diffusion geometry.
PG  - 2858-72
LID - 10.1111/j.1462-2920.2010.02267.x [doi]
AB  - The link between nitritation success in a membrane-aerated biofilm reactor (MABR) 
      and the composition of the initial ammonia- and nitrite-oxidizing bacterial (AOB 
      and NOB) population was investigated. Four identically operated flat-sheet type 
      MABRs were initiated with two different inocula: from an autotrophic nitrifying 
      bioreactor (Inoculum A) or from a municipal wastewater treatment plant (Inoculum 
      B). Higher nitritation efficiencies (NO(2)(-)-N/NH(4)(+)-N) were obtained in the 
      Inoculum B- (55.2-56.4%) versus the Inoculum A- (20.2-22.1%) initiated reactors. 
      The biofilms had similar oxygen penetration depths (100-150 microm), but the AOB 
      profiles [based on 16S rRNA gene targeted real-time quantitative PCR (qPCR)] 
      revealed different peak densities at or distant from the membrane surface in the 
      Inoculum B- versus A-initiated reactors, respectively. Quantitative fluorescence 
      in situ hybridization (FISH) revealed that the predominant AOB in the Inoculum A- 
      and B-initiated reactors were Nitrosospira spp. (48.9-61.2%) versus halophilic 
      and halotolerant Nitrosomonas spp. (54.8-63.7%), respectively. The latter biofilm 
      displayed a higher specific AOB activity than the former biofilm (1.65 fmol 
      cell(-1) h(-1) versus 0.79 fmol cell(-1) h(-1) ). These observations suggest that 
      the AOB and NOB population compositions of the inoculum may determine dominant 
      AOB in the MABR biofilm, which in turn affects the degree of attainable 
      nitritation in an MABR.
CI  - (c) 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
FAU - Terada, Akihiko
AU  - Terada A
AD  - Department of Environmental Engineering, Technical University of Denmark, DK-2800 
      Lyngby, Denmark.
FAU - Lackner, Susanne
AU  - Lackner S
FAU - Kristensen, Ken
AU  - Kristensen K
FAU - Smets, Barth F
AU  - Smets BF
LA  - eng
SI  - GENBANK/FJ529918
SI  - GENBANK/FJ529919
SI  - GENBANK/FJ529920
SI  - GENBANK/FJ710745
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Nitrites)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/metabolism
MH  - Autotrophic Processes
MH  - Bacteria/*classification/genetics/metabolism
MH  - Base Sequence
MH  - *Biodiversity
MH  - Biofilms/*classification/growth & development
MH  - Bioreactors/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Nitrification
MH  - Nitrites/metabolism
MH  - Nitrosomonas/classification/growth & development/metabolism
MH  - RNA, Ribosomal, 16S
EDAT- 2010/06/16 06:00
MHDA- 2011/02/01 06:00
CRDT- 2010/06/16 06:00
PHST- 2010/06/16 06:00 [entrez]
PHST- 2010/06/16 06:00 [pubmed]
PHST- 2011/02/01 06:00 [medline]
AID - EMI2267 [pii]
AID - 10.1111/j.1462-2920.2010.02267.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2010 Oct;12(10):2858-72. doi: 
      10.1111/j.1462-2920.2010.02267.x.