PMID- 20546240
OWN - NLM
STAT- MEDLINE
DCOM- 20100629
LR  - 20211020
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 43
IP  - 3
DP  - 2010 Jun
TI  - Multilineage differentiation of dental follicle cells and the roles of Runx2 
      over-expression in enhancing osteoblast/cementoblast-related gene expression in 
      dental follicle cells.
PG  - 219-28
LID - 10.1111/j.1365-2184.2010.00670.x [doi]
AB  - OBJECTIVES: Dental follicle cells (DFCs) provide the origin of periodontal 
      tissues, and Runx2 is essential for bone formation and tooth development. In this 
      study, pluripotency of DFCs was evaluated and effects of Runx2 on them were 
      investigated. MATERIALS AND METHODS: The DFCs were induced to differentiate 
      towards osteoblasts, adipocytes or chondrocytes, and alizarin red staining, oil 
      red O staining or alcian blue staining was performed to reveal the differentiated 
      states. Bone marrow stromal cells (BMSCs) and primary mouse fibroblasts served as 
      controls. DFCs were also infected with recombinant retroviruses encoding either 
      full-length Runx2 or mutant Runx2 without the VWRPY motif. Western blot analysis, 
      real-time real time RT-PCR and in vitro mineralization assay were performed to 
      evaluate the effects of full-length Runx2 or mutant Runx2 on 
      osteogenic/cementogenic differentiation of the cells. RESULTS: The 
      above-mentioned staining methods demonstrated that DFCs were successfully induced 
      to differentiate towards osteoblasts, adipocytes or chondrocytes respectively, 
      confirming the existence of pluripotent mesenchymal stem cells in dental follicle 
      tissues. However, staining intensity in DFC cultures was weaker than in BMSC 
      cultures. Real-time PCR analysis indicated that mutant Runx2 induced a more 
      pronounced increase in expression levels of OC, OPN, Col I and CP23 than 
      full-length Runx2. Mineralization assay also showed that mutant Runx2 increased 
      mineralization nodule formation more prominently than full-length Runx2. 
      CONCLUSIONS: Multipotent DFCs can be induced to differentiate towards 
      osteoblasts, adipocytes or chondrocytes in vitro. Runx2 over-expression 
      up-regulated expression levels of osteoblast/cementoblast-related genes and in 
      vitro enhanced osteogenic differentiation of DFCs. In addition, mutant 
      Runx2-induced changes in DFCs were more prominent than those induced by 
      full-length Runx2.
FAU - Pan, K
AU  - Pan K
AD  - Department of Periodontology and Institute of Oral Biomedicine, School of 
      Dentistry, Shandong University, Jinan, China.
FAU - Sun, Q
AU  - Sun Q
FAU - Zhang, J
AU  - Zhang J
FAU - Ge, S
AU  - Ge S
FAU - Li, S
AU  - Li S
FAU - Zhao, Y
AU  - Zhao Y
FAU - Yang, P
AU  - Yang P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Runx2 protein, mouse)
SB  - IM
MH  - Adipocytes/cytology/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation/genetics
MH  - Cell Lineage/genetics
MH  - Chondrocytes/cytology/metabolism
MH  - Core Binding Factor Alpha 1 Subunit/*genetics
MH  - Dental Cementum/cytology/metabolism
MH  - Dental Sac/cytology/*growth & development
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mutation/genetics
MH  - NIH 3T3 Cells
MH  - Osteoblasts/cytology/metabolism
MH  - Pluripotent Stem Cells/cytology/*metabolism
MH  - Tooth/cytology/*growth & development
MH  - Transfection
PMC - PMC6496149
EDAT- 2010/06/16 06:00
MHDA- 2010/06/30 06:00
PMCR- 2010/04/28
CRDT- 2010/06/16 06:00
PHST- 2010/06/16 06:00 [entrez]
PHST- 2010/06/16 06:00 [pubmed]
PHST- 2010/06/30 06:00 [medline]
PHST- 2010/04/28 00:00 [pmc-release]
AID - CPR670 [pii]
AID - 10.1111/j.1365-2184.2010.00670.x [doi]
PST - ppublish
SO  - Cell Prolif. 2010 Jun;43(3):219-28. doi: 10.1111/j.1365-2184.2010.00670.x.