PMID- 20546256
OWN - NLM
STAT- MEDLINE
DCOM- 20101018
LR  - 20161125
IS  - 1464-5491 (Electronic)
IS  - 0742-3071 (Linking)
VI  - 27
IP  - 2
DP  - 2010 Feb
TI  - Polymorphisms of the macrophage migration inhibitory factor gene in a UK 
      population with Type 1 diabetes mellitus.
PG  - 143-9
LID - 10.1111/j.1464-5491.2009.02916.x [doi]
AB  - AIMS: Macrophage migration inhibitory factor (MIF) is a potent pro-inflammatory 
      cytokine whose production is transcriptionally regulated by glucose. Experimental 
      data from both Type 1 diabetes mellitus (T1D) patients and animal models suggests 
      a role for MIF in the development of T1D. The aim of this study was to employ 
      gene resequencing to identify common DNA polymorphisms in the MIF gene and 
      subsequently assess haplotype tagged single nucleotide polymorphisms (htSNPs) 
      using a combination of case-control and family-based association analyses in 
      order to assess the association of MIF htSNPs with the development of T1D in a 
      white population. METHODS: All exons, introns and approximately 3 kb upstream and 
      downstream of the MIF gene were screened for DNA polymorphisms in 46 individuals 
      using DNA sequencing. Genotyping of the htSNPs was performed in 432 cases, 407 
      control subjects and 290 T1D parent-offspring trios, using Taqman, Sequenom, 
      Pyrosequencing and fluorescence-based microsatellite technologies. RESULTS: 
      Twenty-three polymorphisms (two novel) with a minor allele frequency > 10% were 
      identified. Four MIF htSNPs (rs875643 G>A, rs7388067 C>T, rs5844572 -/CATT, 
      rs6003941 T>G) were identified. Allele and haplotype frequencies were similar 
      between case and control groups (P > 0.6 by permutation test) and assessment of 
      allele transmission distortion from informative parents to affected offspring 
      also failed to find an association. Stratification of these analyses for 
      age-at-onset and human leukocyte antigen (HLA)-DR risk group (DR3/DR4) did not 
      reveal any significant associations. CONCLUSIONS: It is unlikely that common 
      polymorphisms in the MIF gene strongly influence susceptibility to T1D in the UK 
      population.
FAU - Martin, R J L
AU  - Martin RJ
AD  - Nephrology, Queen's University of Belfast, Belfast, UK. rosalind.martin@qub.ac.uk
FAU - Savage, D A
AU  - Savage DA
FAU - Carson, D J
AU  - Carson DJ
FAU - McKnight, A J
AU  - McKnight AJ
FAU - Maxwell, A P
AU  - Maxwell AP
FAU - Patterson, C C
AU  - Patterson CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Diabet Med
JT  - Diabetic medicine : a journal of the British Diabetic Association
JID - 8500858
RN  - 0 (Macrophage Migration-Inhibitory Factors)
SB  - IM
MH  - Adolescent
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 1/*genetics
MH  - Family
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Haplotypes
MH  - Humans
MH  - Introns
MH  - Linkage Disequilibrium
MH  - Macrophage Migration-Inhibitory Factors/*genetics
MH  - Male
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Sequence Analysis, DNA
MH  - United Kingdom
EDAT- 2010/06/16 06:00
MHDA- 2010/10/19 06:00
CRDT- 2010/06/16 06:00
PHST- 2010/06/16 06:00 [entrez]
PHST- 2010/06/16 06:00 [pubmed]
PHST- 2010/10/19 06:00 [medline]
AID - DME2916 [pii]
AID - 10.1111/j.1464-5491.2009.02916.x [doi]
PST - ppublish
SO  - Diabet Med. 2010 Feb;27(2):143-9. doi: 10.1111/j.1464-5491.2009.02916.x.