PMID- 20547241
OWN - NLM
STAT- MEDLINE
DCOM- 20101101
LR  - 20161125
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 42
IP  - 9
DP  - 2010 Sep
TI  - Transcriptional regulation and functional implication of the grass carp CITED1 
      (gcCITED1) in the negative regulation of HIF-1.
PG  - 1544-52
LID - 10.1016/j.biocel.2010.06.007 [doi]
AB  - Hypoxia triggers a broad range of gene responses that are primarily mediated by 
      the transcription factor, hypoxia-inducible factor-1 (HIF-1) that complexes with 
      the transcriptional coactivator CREB-binding protein/p300 (CBP/p300). In mammals, 
      members of the CBP/p300-interacting transactivators with ED-rich tail (CITED) 
      family, such as CITED2 and CITED4, bind CBP/p300 with high affinity and thereby 
      negatively regulate HIF-1 transactivation. In fish, we have previously shown that 
      two CITED3 homologues from the hypoxia-tolerant grass carp (Ctenopharyngodon 
      idellus) are induced by hypoxia/HIF-1 and able to inhibit HIF-1 transactivation. 
      Here we report the identification and functional characterization of the grass 
      carp CITED1 (gcCITED1) protein as a new repressor of HIF-1-mediated 
      transcriptional activity. Expression of gcCITED1 mRNA was increased in heart, 
      kidney and liver in vivo after exposure to hypoxia. Luciferase reporter and ChIP 
      assays, respectively, indicated the inducibility of the gcCITED1 promoter by 
      gcHIF-1 and the in vivo binding of gcHIF-1 to the gcCITED1 promoter. Ectopic 
      overexpression of gcCITED1 significantly attenuated HIF-1-dependent 
      transactivation of a HRE-luciferase reporter gene. Furthermore, GST pull-down 
      confirmed that gcCITED1 specifically binds via its CR2 domain to the CH1 region 
      of the grass carp p300 coactivator. Overall, our findings suggest that the 
      hypoxia/gcHIF-1-inducible gcCITED1 may function in a negative feedback loop to 
      regulate gcHIF-1 activity in response to hypoxia stress.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Ng, Patrick Kwok Shing
AU  - Ng PK
AD  - Department of Biochemistry, Chinese University of Hong Kong, New Territories, 
      Hong Kong Special Administrative Region, China.
FAU - Yu, Richard Man Kit
AU  - Yu RM
FAU - Kwong, Theresa Fuk Ning
AU  - Kwong TF
FAU - Wong, Minnie Man Lai
AU  - Wong MM
FAU - Kong, Richard Yuen Chong
AU  - Kong RY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100612
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Fish Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Carps/classification/genetics/*metabolism
MH  - Chromatin Immunoprecipitation
MH  - Fish Proteins/chemistry/genetics/*metabolism
MH  - *Gene Expression Regulation
MH  - Hypoxia/genetics
MH  - Hypoxia-Inducible Factor 1/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Promoter Regions, Genetic/genetics
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/chemistry/genetics/*metabolism
EDAT- 2010/06/16 06:00
MHDA- 2010/11/03 06:00
CRDT- 2010/06/16 06:00
PHST- 2010/04/09 00:00 [received]
PHST- 2010/06/07 00:00 [revised]
PHST- 2010/06/07 00:00 [accepted]
PHST- 2010/06/16 06:00 [entrez]
PHST- 2010/06/16 06:00 [pubmed]
PHST- 2010/11/03 06:00 [medline]
AID - S1357-2725(10)00204-9 [pii]
AID - 10.1016/j.biocel.2010.06.007 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2010 Sep;42(9):1544-52. doi: 
      10.1016/j.biocel.2010.06.007. Epub 2010 Jun 12.