PMID- 20547545
OWN - NLM
STAT- MEDLINE
DCOM- 20101123
LR  - 20211020
IS  - 1460-2377 (Electronic)
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 22
IP  - 8
DP  - 2010 Aug
TI  - I-Ag7 is subject to post-translational chaperoning by CLIP.
PG  - 705-16
LID - 10.1093/intimm/dxq056 [doi]
AB  - Several MHC class II alleles linked with autoimmune diseases form unusually 
      low-stability complexes with class II-associated invariant chain peptides (CLIP), 
      leading us to hypothesize that this is an important feature contributing to 
      autoimmune pathogenesis. We recently demonstrated a novel post-endoplasmic 
      reticulum (ER) chaperoning role of the CLIP peptides for the murine class II 
      allele I-E(d). In the current study, we tested the generality of this CLIP 
      chaperone function using a series of invariant chain (Ii) mutants designed to 
      have varying CLIP affinity for I-A(g7). In cells expressing these Ii CLIP 
      mutants, I-A(g7) abundance, turnover and antigen presentation are all subject to 
      regulation by CLIP affinity, similar to I-E(d). However, I-A(g7) undergoes much 
      greater quantitative changes than observed for I-E(d). In addition, we find that 
      Ii with a CLIP region optimized for I-A(g7) binding may be preferentially 
      assembled with I-A(g7) even in the presence of higher levels of wild-type Ii. 
      This finding indicates that, although other regions of Ii interact with class II, 
      CLIP binding to the groove is likely to be a dominant event in assembly of 
      nascent class II molecules with Ii in the ER.
FAU - Rinderknecht, Cornelia H
AU  - Rinderknecht CH
AD  - Program in Immunology, Stanford University, Stanford, CA 94305, USA.
FAU - Lu, Ning
AU  - Lu N
FAU - Crespo, Oliver
AU  - Crespo O
FAU - Truong, Phi
AU  - Truong P
FAU - Hou, Tieying
AU  - Hou T
FAU - Wang, Nan
AU  - Wang N
FAU - Rajasekaran, Narendiran
AU  - Rajasekaran N
FAU - Mellins, Elizabeth D
AU  - Mellins ED
LA  - eng
GR  - T32 AI007290/AI/NIAID NIH HHS/United States
GR  - T32 AI07290/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100613
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Antigens, Surface)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (I-A g7 antigen)
RN  - 0 (Molecular Chaperones)
RN  - 0 (invariant chain)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation, B-Lymphocyte/genetics/*immunology
MH  - Antigens, Surface/genetics/immunology
MH  - B-Lymphocytes/immunology
MH  - Cell Line, Tumor
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class II/genetics/*immunology
MH  - Immunoblotting
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Chaperones/*immunology
MH  - Mutation
MH  - Protein Processing, Post-Translational/*immunology
PMC - PMC2908477
EDAT- 2010/06/16 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/08/01
CRDT- 2010/06/16 06:00
PHST- 2010/06/16 06:00 [entrez]
PHST- 2010/06/16 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/08/01 00:00 [pmc-release]
AID - dxq056 [pii]
AID - 10.1093/intimm/dxq056 [doi]
PST - ppublish
SO  - Int Immunol. 2010 Aug;22(8):705-16. doi: 10.1093/intimm/dxq056. Epub 2010 Jun 13.