PMID- 20549975
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120105
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 3
DP  - 2010 Mar
TI  - [Rapid analysis of trace levels of riboflavin and its derivatives with 
      hydrophilic interaction monolith by pressurized capillary electrochromatography 
      with laser induced fluorescence detection].
PG  - 247-52
AB  - A novel hydrophilic interaction capillary electrochromatographic (HI-CEC) 
      monolith with covalently bonded zwitterionic functional groups was applied for 
      the separation of riboflavins (RF) and its derivatives. With a homemade 
      pressurized capillary electrochromatography-laser induced fluorescence detection 
      (pCEC-LIF) system, trace levels of RF and its derivatives, flavin mononucleotide 
      (FMN) and flavin adenine dinucleotide (FAD), can be baseline separated within 8.0 
      min in isocratic elution mode. The effect of experimental parameters on 
      separation was investigated. Under the optimum conditions, analytes could be 
      determined over nearly three orders of magnitudes with the detection limits 
      (LODs) as low as 5.0 x 10(-11) mol/L (RF), 8.0 x 10(-10) mol/L (FMN), 2.5 x 
      10(-9) mol/L (FAD), and the relative standard deviations (RSDs) were less than 
      8.2%. This method is rapid, simple, repeatable and more sensitive than the most 
      of reported methods, and satisfied results has been achieved in serum sample. 
      Furthermore, it can be further applied for trace analysis of RF and its 
      derivatives in biological fluid and cells.
FAU - Wu, Yimin
AU  - Wu Y
AD  - College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, 
      China.
FAU - Wu, Qingzheng
AU  - Wu Q
FAU - Wang, Xiaochun
AU  - Wang X
FAU - Xie, Zenghong
AU  - Xie Z
FAU - Wu, Xiaoping
AU  - Wu X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
EDAT- 2010/06/17 06:00
MHDA- 2010/06/17 06:01
CRDT- 2010/06/17 06:00
PHST- 2010/06/17 06:00 [entrez]
PHST- 2010/06/17 06:00 [pubmed]
PHST- 2010/06/17 06:01 [medline]
AID - 10.3724/sp.j.1123.2010.00247 [doi]
PST - ppublish
SO  - Se Pu. 2010 Mar;28(3):247-52. doi: 10.3724/sp.j.1123.2010.00247.