PMID- 20549982
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 3
DP  - 2010 Mar
TI  - [Separation of proteins on microchip electrophoresis and its comparison with DNA 
      migration].
PG  - 296-300
AB  - The efficient separation of six standard proteins on a home-made poly 
      (dimethylsiloxane) microchip with an auto-deducting background diode laser 
      induced fluorescence detector was accomplished within 6.4 min under the sieving 
      matrix of 10 g/L hydroxyethyl cellulose (HEC), 1 g/L sodium dodecyl sulphonate 
      (SDS), 40 mmol/L phosphate buffer at pH 7.0. The experimental results showed that 
      the reproducibility of protein separation was satisfactory and the relative 
      standard deviations (RSDs) of protein migration time were less than 10%. The 
      migration times of the proteins are analyzed by a quantitative mathematical model 
      of deoxyribonucleic acid (DNA) proposed by ourselves previously. The results 
      showed that the migration character of SDS-protein complexes was similar with 
      DNA. However, the linear relationships between the mobilities of SDS-protein 
      complexes and their relative molecular mass as well as electric field strength 
      became worse, which indicated the mathematical model for DNA separation should be 
      revised before it is used for protein separation.
FAU - Liu, Chunye
AU  - Liu C
AD  - School of Pharmacy, Xi'an Medical University, Xi'an 710021, China. 
      doris8976@163.com
FAU - Xu, Xu
AU  - Xu X
FAU - Zhang, Jian
AU  - Zhang J
FAU - Chen, Jierong
AU  - Chen J
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Nylons)
RN  - 0 (Proteins)
RN  - 0 (poly(dimethylsiloxane)-polyamide copolymer)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*isolation & purification
MH  - Dimethylpolysiloxanes/chemistry
MH  - Electrophoresis, Microchip/*methods
MH  - *Emigration and Immigration
MH  - Models, Theoretical
MH  - Nylons/chemistry
MH  - Proteins/*isolation & purification
EDAT- 2010/06/17 06:00
MHDA- 2012/01/06 06:00
CRDT- 2010/06/17 06:00
PHST- 2010/06/17 06:00 [entrez]
PHST- 2010/06/17 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 10.3724/sp.j.1123.2010.00296 [doi]
PST - ppublish
SO  - Se Pu. 2010 Mar;28(3):296-300. doi: 10.3724/sp.j.1123.2010.00296.