PMID- 20550687
OWN - NLM
STAT- MEDLINE
DCOM- 20140714
LR  - 20211020
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 1
IP  - 2
DP  - 2010 Jun 15
TI  - Homing of stem cells to sites of inflammatory brain injury after intracerebral 
      and intravenous administration: a longitudinal imaging study.
PG  - 17
LID - 10.1186/scrt17 [doi]
AB  - INTRODUCTION: This study aimed to determine the homing potential and fate of 
      epidermal neural crest stem cells (eNCSCs) derived from hair follicles, and bone 
      marrow-derived stem cells (BMSCs) of mesenchymal origin, in a lipopolysaccharide 
      (LPS)-induced inflammatory lesion model in the rat brain. Both eNCSCs and BMSCs 
      are easily accessible from adult tissues by using minimally invasive procedures 
      and can differentiate into a variety of neuroglial lineages. Thus, these cells 
      have the potential to be used in autologous cell-replacement therapies, 
      minimizing immune rejection, and engineered to secrete a variety of molecules. 
      METHODS: Both eNCSCs and BMSCs were prelabeled with iron-oxide nanoparticles 
      (IO-TAT-FITC) and implanted either onto the corpus callosum in healthy or 
      LPS-lesioned animals or intravenously into lesioned animals. Both cell types were 
      tracked longitudinally in vivo by using magnetic resonance imaging (MRI) for up 
      to 30 days and confirmed by postmortem immunohistochemistry. RESULTS: 
      Transplanted cells in nonlesioned animals remained localized along the corpus 
      callosum. Cells implanted distally from an LPS lesion (either intracerebrally or 
      intravenously) migrated only toward the lesion, as seen by the localized MRI 
      signal void. Fluorescence microscopy of the FITC tag on the nanoparticles 
      confirmed the in vivo MRI data, CONCLUSIONS: This study demonstrated that both 
      cell types can be tracked in vivo by using noninvasive MRI and have pathotropic 
      properties toward an inflammatory lesion in the brain. As these cells 
      differentiate into the glial phenotype and are derived from adult tissues, they 
      offer a viable alternative autologous stem cell source and gene-targeting 
      potential for neurodegenerative and demyelinating pathologies.
FAU - Jackson, Johanna S
AU  - Jackson JS
AD  - Stem Cell Imaging, MRC Clinical Sciences Centre, Imperial College London, Du Cane 
      Road, London W12 0NN, UK. johanna.jackson@med.lu.se
FAU - Golding, Jon P
AU  - Golding JP
FAU - Chapon, Catherine
AU  - Chapon C
FAU - Jones, William A
AU  - Jones WA
FAU - Bhakoo, Kishore K
AU  - Bhakoo KK
LA  - eng
GR  - G113/31/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100615
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (Ferric Compounds)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (SOXE Transcription Factors)
RN  - 0 (Sox10 protein, rat)
RN  - 1K09F3G675 (ferric oxide)
SB  - IM
MH  - Administration, Intravenous
MH  - Animals
MH  - Bone Marrow Cells
MH  - Brain/cytology/pathology
MH  - Brain Injuries/chemically induced/*therapy
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cell- and Tissue-Based Therapy
MH  - Corpus Callosum/*metabolism
MH  - Demyelinating Diseases/therapy
MH  - Ferric Compounds
MH  - Hair Follicle/cytology
MH  - Lipopolysaccharides
MH  - Magnetic Resonance Imaging
MH  - Metal Nanoparticles
MH  - Microscopy, Fluorescence
MH  - Neural Crest/cytology
MH  - Neurodegenerative Diseases/therapy
MH  - Neuroglia/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - SOXE Transcription Factors/metabolism
MH  - *Stem Cell Transplantation
MH  - Stem Cells/*metabolism
MH  - Transplantation, Autologous
PMC - PMC2905093
EDAT- 2010/06/17 06:00
MHDA- 2010/06/17 06:01
PMCR- 2010/06/15
CRDT- 2010/06/17 06:00
PHST- 2010/05/14 00:00 [received]
PHST- 2010/06/15 00:00 [accepted]
PHST- 2010/06/17 06:00 [entrez]
PHST- 2010/06/17 06:00 [pubmed]
PHST- 2010/06/17 06:01 [medline]
PHST- 2010/06/15 00:00 [pmc-release]
AID - scrt17 [pii]
AID - 10.1186/scrt17 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2010 Jun 15;1(2):17. doi: 10.1186/scrt17.