PMID- 20551436
OWN - NLM
STAT- MEDLINE
DCOM- 20110105
LR  - 20211020
IS  - 1943-2631 (Electronic)
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 186
IP  - 1
DP  - 2010 Sep
TI  - QIP, a protein that converts duplex siRNA into single strands, is required for 
      meiotic silencing by unpaired DNA.
PG  - 119-26
LID - 10.1534/genetics.110.118273 [doi]
AB  - RNA interference (RNAi) depends on the production of small RNA to regulate gene 
      expression in eukaryotes. Two RNAi systems exist to control repetitive selfish 
      elements in Neurospora crassa. Quelling targets transgenes during vegetative 
      growth, whereas meiotic silencing by unpaired DNA (MSUD) silences unpaired genes 
      during meiosis. The two mechanisms require common RNAi proteins, such as 
      RNA-directed RNA polymerases, Dicers, and Argonaute slicers. We have previously 
      demonstrated that, while Quelling depends on the redundant dicer activity of 
      DCL-1 and DCL-2, only DCL-1 is required for MSUD. Here, we show that 
      QDE-2-interacting protein (QIP), an exonuclease that is important for the 
      production of single-stranded siRNA during Quelling, is also required for MSUD. 
      QIP is crucial for sexual development and is shown to colocalize with other MSUD 
      proteins in the perinuclear region.
FAU - Xiao, Hua
AU  - Xiao H
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211, USA.
FAU - Alexander, William G
AU  - Alexander WG
FAU - Hammond, Thomas M
AU  - Hammond TM
FAU - Boone, Erin C
AU  - Boone EC
FAU - Perdue, Tony D
AU  - Perdue TD
FAU - Pukkila, Patricia J
AU  - Pukkila PJ
FAU - Shiu, Patrick K T
AU  - Shiu PK
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100615
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.1.- (Exonucleases)
SB  - IM
MH  - Cell Nucleus/metabolism
MH  - DNA, Single-Stranded/*genetics
MH  - Exonucleases/genetics/*metabolism
MH  - Gene Expression Regulation, Fungal
MH  - *Gene Silencing
MH  - Homozygote
MH  - Meiosis/*genetics
MH  - Neurospora crassa/cytology/enzymology/*genetics/growth & development
MH  - Protein Transport
MH  - RNA, Double-Stranded/*metabolism
MH  - RNA, Small Interfering/genetics/*metabolism
PMC - PMC2940280
EDAT- 2010/06/17 06:00
MHDA- 2011/01/06 06:00
PMCR- 2011/09/01
CRDT- 2010/06/17 06:00
PHST- 2010/06/17 06:00 [entrez]
PHST- 2010/06/17 06:00 [pubmed]
PHST- 2011/01/06 06:00 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - genetics.110.118273 [pii]
AID - gen1861119 [pii]
AID - 10.1534/genetics.110.118273 [doi]
PST - ppublish
SO  - Genetics. 2010 Sep;186(1):119-26. doi: 10.1534/genetics.110.118273. Epub 2010 Jun 
      15.