PMID- 20551614
OWN - NLM
STAT- MEDLINE
DCOM- 20100806
LR  - 20220317
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 129
IP  - 1-3
DP  - 2010 Jul
TI  - Karyotyping in melon (Cucumis melo L.) by cross-species fosmid fluorescence in 
      situ hybridization.
PG  - 241-9
LID - 10.1159/000314343 [doi]
AB  - Chromosome identification is critical for cytogenetic research and will 
      accelerate studies on genetic variation and breeding, especially for those 
      species with relatively little sequence information. So far, no reliable 
      cytological landmarks have been developed to distinguish individual chromosomes 
      in melon. In this study, using FISH (fluorescence in situ hybridization) combined 
      with comparative genome information, we selected 21 cucumber fosmids anchored by 
      SSR markers as chromosome-specific cytological markers for melon chromosomes. 
      Moreover, with the help of melon centromeric satellite DNA repeats CentM, 45S 
      rDNA and 5S rDNA, sequential FISH with 3 sets of multi-fosmid cocktails were 
      conducted on the same metaphase cell, which allowed us to simultaneously identify 
      each of the 12 metaphase chromosomes of melon and a standardized melon karyotype 
      of somatic metaphase chromosomes was constructed. Finally, we compared the 
      distribution of 21 FISH-mapped fosmids between melon and cucumber chromosomes, 
      which allows a better understanding of the evolutionary process shaping these 2 
      species. Our study provides a basis for cytological characterization of the melon 
      genome and comparative genomics of Cucurbitaceae.
CI  - Copyright 2010 S. Karger AG, Basel.
FAU - Liu, C
AU  - Liu C
AD  - National Maize Improvement Center of China, Key Laboratory of Crop Genetic 
      Improvement and Genome of the Ministry of Agriculture, Beijing Key Laboratory of 
      Crop Genetic Improvement, China Agricultural University, Beijing, China.
FAU - Liu, J
AU  - Liu J
FAU - Li, H
AU  - Li H
FAU - Zhang, Z
AU  - Zhang Z
FAU - Han, Y
AU  - Han Y
FAU - Huang, S
AU  - Huang S
FAU - Jin, W
AU  - Jin W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100611
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Plant/genetics
MH  - Cucumis melo/*genetics
MH  - Cucumis sativus/genetics
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal/genetics
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Minisatellite Repeats
MH  - Species Specificity
EDAT- 2010/06/17 06:00
MHDA- 2010/08/07 06:00
CRDT- 2010/06/17 06:00
PHST- 2010/06/17 06:00 [entrez]
PHST- 2010/06/17 06:00 [pubmed]
PHST- 2010/08/07 06:00 [medline]
AID - 000314343 [pii]
AID - 10.1159/000314343 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2010 Jul;129(1-3):241-9. doi: 10.1159/000314343. Epub 2010 
      Jun 11.