PMID- 20551615
OWN - NLM
STAT- MEDLINE
DCOM- 20100806
LR  - 20100714
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 129
IP  - 1-3
DP  - 2010 Jul
TI  - Fate of Aegilops speltoides-derived, repetitive DNA sequences in diploid Aegilops 
      species, wheat-Aegilops amphiploids and derived chromosome addition lines.
PG  - 47-54
LID - 10.1159/000314552 [doi]
AB  - The present study reports the cloning and characterization of an Aegilops 
      speltoides-derived subtelomeric repeat, designated as pSp1B16. Clone pSp1B16 has 
      98% sequence homology with the previously isolated Ae. speltoides repeat Spelt1. 
      The distribution of pSp1B16 and another Ae. speltoides repeat, pGc1R1, was 
      analyzed in diploid Aegilops species, tetra- and hexaploid wheats, wheat-Aegilops 
      amphiploids and derived chromosome addition lines by fluorescence in situ 
      hybridization (FISH). Clones pSp1B16 and pGc1R1 revealed FISH sites in Ae. 
      speltoides, Ae. sharonensis and Triticum timopheevii, whereas additional pGc1R1 
      FISH sites were observed in Ae. longissima and Ae. caudata. The pSp1B16 and 
      pGc1R1 FISH patterns of the Aegilops chromosomes in the wheat-Aegilops 
      amphiploids and chromosome addition lines are similar to those present in the 
      Aegilops parent accession. We did not observe any evidence of pSp1B16 and pGc1R1 
      sequence elimination, which is in contrast to previous studies using similar 
      hybrids and repeats. The presented data suggest that the genomic changes in 
      synthetic amphiploids observed in previous studies might be caused by 
      homoeologous recombination, which was suppressed in the amphiploid analyzed in 
      this study.
CI  - Copyright 2010 S. Karger AG, Basel.
FAU - Kumar, S
AU  - Kumar S
AD  - Molecular Cytogenetics Laboratory, Department of Molecular Biology and Genetic 
      Engineering, College of Basic Sciences and Humanities, G. B. Pant University of 
      Agriculture and Technology, Uttarakhand, India.
FAU - Friebe, B
AU  - Friebe B
FAU - Gill, B S
AU  - Gill BS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100615
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - Cloning, Molecular
MH  - DNA, Plant/*genetics
MH  - Diploidy
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Poaceae/classification/*genetics
MH  - Polyploidy
MH  - Recombination, Genetic
MH  - Species Specificity
MH  - Tandem Repeat Sequences
MH  - Triticum/classification/*genetics
EDAT- 2010/06/17 06:00
MHDA- 2010/08/07 06:00
CRDT- 2010/06/17 06:00
PHST- 2010/06/17 06:00 [entrez]
PHST- 2010/06/17 06:00 [pubmed]
PHST- 2010/08/07 06:00 [medline]
AID - 000314552 [pii]
AID - 10.1159/000314552 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2010 Jul;129(1-3):47-54. doi: 10.1159/000314552. Epub 2010 
      Jun 15.