PMID- 20553723
OWN - NLM
STAT- MEDLINE
DCOM- 20101210
LR  - 20131121
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 168
IP  - 3
DP  - 2010 Sep 15
TI  - Tight junction proteins in zebrafish ovarian follicles: stage specific mRNA 
      abundance and response to 17beta-estradiol, human chorionic gonadotropin, and 
      maturation inducing hormone.
PG  - 388-400
LID - 10.1016/j.ygcen.2010.05.011 [doi]
AB  - In vertebrate epithelia, the tight junction (TJ) complex plays an important role 
      in the regulation of paracellular permeability and contributes to mechanical 
      stability. Using zebrafish, this study examined the possibility that TJ protein 
      'machinery' may contribute to the complex process of ovarian follicle development 
      in fishes and be responsive to key endocrine factors that assist in the 
      regulation of this event. Transcript encoding for 18 zebrafish claudin (cldn) and 
      2 occludin (ocln) orthologs were widely distributed in zebrafish tissues. All 
      orthologs were detected in the ovary, albeit at varying levels of abundance. 
      Using occludin as a marker, TJs localized to the periphery of ovarian follicles, 
      corresponding to the position of granulosa and theca cells. Of the 20 orthologs 
      examined, mRNA encoding for cldn g, d, and ocln were most abundant in whole 
      ovary. As follicles transitioned from pre-vitellogenic to mid-/late-stage 
      vitellogenic status, mRNA encoding for cldn a, b, d, g, h and 19 significantly 
      declined. Transcript encoding for other orthologs (i.e. cldn c, e, f, i, j, 2, 7, 
      8, 10, 11, 12, 15, ocln and ocln b) did not significantly alter during follicle 
      development. Exogenous 17beta-estradiol had little effect on TJ machinery in 
      pre-vitellogenic follicles, but reduced the abundance of cldn h and 10 in mid- to 
      late-stage vitellogenic follicles. In mid-vitellogenic follicles human chorionic 
      gonadotropin increased cldn d and g mRNA abundance, whereas maturation inducing 
      hormone reduced cldn h and 19 mRNA abundance. The data supports a role for the 
      endocrine regulation of TJ proteins in ovarian follicle development.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Clelland, Eric S
AU  - Clelland ES
AD  - Department of Biology, York University, Toronto, Ontario, Canada. 
      clelland@yorku.ca
FAU - Kelly, Scott P
AU  - Kelly SP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100527
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Zebrafish Proteins)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Chorionic Gonadotropin/*pharmacology
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Humans
MH  - Oocytes/drug effects/metabolism
MH  - Ovarian Follicle/*drug effects/*metabolism
MH  - RNA, Messenger/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tight Junctions/*metabolism
MH  - Zebrafish
MH  - Zebrafish Proteins/*genetics
EDAT- 2010/06/18 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/06/18 06:00
PHST- 2010/03/30 00:00 [received]
PHST- 2010/05/11 00:00 [revised]
PHST- 2010/05/23 00:00 [accepted]
PHST- 2010/06/18 06:00 [entrez]
PHST- 2010/06/18 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0016-6480(10)00216-9 [pii]
AID - 10.1016/j.ygcen.2010.05.011 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2010 Sep 15;168(3):388-400. doi: 
      10.1016/j.ygcen.2010.05.011. Epub 2010 May 27.