PMID- 20554771
OWN - NLM
STAT- MEDLINE
DCOM- 20100916
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 84
IP  - 17
DP  - 2010 Sep
TI  - Phylogeny-based evolutionary, demographical, and geographical dissection of North 
      American type 2 porcine reproductive and respiratory syndrome viruses.
PG  - 8700-11
LID - 10.1128/JVI.02551-09 [doi]
AB  - Type 2 (or North American-like) porcine reproductive and respiratory syndrome 
      virus (PRRSV) was first recorded in 1987 in the United States and now occurs in 
      most commercial swine industries throughout the world. In this study, we 
      investigated the epidemiological and evolutionary behaviors of type 2 PRRSV. 
      Based on phylogenetic analyses of 8,624 ORF5 sequences, we described a 
      comprehensive picture of the diversity of type 2 PRRSVs and systematically 
      classified all available sequences into lineages and sublineages, including a 
      number of previously undescribed lineages. With the rapid growth of sequence 
      deposition into the databases, it would be technically difficult for veterinary 
      researchers to genotype their sequences by reanalyzing all sequences in the 
      databases. To this end, a set of reference sequences was established based on our 
      classification system, which represents the principal diversity of all available 
      sequences and can readily be used for further genotyping studies. In addition, we 
      further investigated the demographic histories of these lineages and sublineages 
      by using Bayesian coalescence analyses, providing evolutionary insights into 
      several important epidemiological events of type 2 PRRSV. Moreover, by using a 
      phylogeographic approach, we were able to estimate the transmission frequencies 
      between the pig-producing states in the United States and identified several 
      states as the major sources of viral spread, i.e., "transmission centers." In 
      summary, this study represents the most extensive phylogenetic analyses of type 2 
      PRRSV to date, providing a basis for future genotyping studies and dissecting the 
      epidemiology of type 2 PRRSV from phylogenetic perspectives.
FAU - Shi, Mang
AU  - Shi M
AD  - School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
FAU - Lam, Tommy Tsan-Yuk
AU  - Lam TT
FAU - Hon, Chung-Chau
AU  - Hon CC
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
FAU - Davies, Peter R
AU  - Davies PR
FAU - Hui, Raymond Kin-Hei
AU  - Hui RK
FAU - Li, Jun
AU  - Li J
FAU - Wong, Lina Tik-Wim
AU  - Wong LT
FAU - Yip, Chi-Wai
AU  - Yip CW
FAU - Jiang, Jin-Wai
AU  - Jiang JW
FAU - Leung, Frederick Chi-Ching
AU  - Leung FC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100616
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
SB  - IM
MH  - Animals
MH  - *Evolution, Molecular
MH  - Genetic Variation
MH  - Geography
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/transmission/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/genetics/isolation & purification
MH  - Swine
PMC - PMC2919017
EDAT- 2010/06/18 06:00
MHDA- 2010/09/18 06:00
PMCR- 2011/03/01
CRDT- 2010/06/18 06:00
PHST- 2010/06/18 06:00 [entrez]
PHST- 2010/06/18 06:00 [pubmed]
PHST- 2010/09/18 06:00 [medline]
PHST- 2011/03/01 00:00 [pmc-release]
AID - JVI.02551-09 [pii]
AID - 2551-09 [pii]
AID - 10.1128/JVI.02551-09 [doi]
PST - ppublish
SO  - J Virol. 2010 Sep;84(17):8700-11. doi: 10.1128/JVI.02551-09. Epub 2010 Jun 16.