PMID- 20555139
OWN - NLM
STAT- MEDLINE
DCOM- 20101102
LR  - 20211020
IS  - 1875-8908 (Electronic)
IS  - 1387-2877 (Print)
IS  - 1387-2877 (Linking)
VI  - 21
IP  - 1
DP  - 2010
TI  - Severe In vivo hyper-homocysteinemia is not associatedwith elevation of 
      amyloid-beta peptides in the Tg2576 mice.
PG  - 133-40
LID - 10.3233/JAD-2010-100171 [doi]
AB  - Since hyper-homocysteinemia (HHcy) was recognized as a risk factor for 
      Alzheimer's disease (AD), many studies tried to induce HHcy in animal models to 
      investigate its effect on amyloid-beta protein precursor (AbetaPP) metabolism. 
      Previous reports found that HHcy induced in AD transgenic mouse models, by either 
      feedina a methionine-enriched diet or vitamin Bs deficient diet, is associated 
      with elevation of amyloid-beta (Abeta) levels. However, there is no data 
      available on the effect of dietary intervention which combines both excessive 
      methionine and low levels of vitamin Bs on amyloidogenesis in any of these 
      models. In the current study, we investigated the effect of a combination diet, 
      which was both enriched in methionine and deficient in folate, vitamin B6 and 
      B12, in an AD mouse model, the Tg2576. We found that 7 months treatment of this 
      diet induced severe HHcy in these mice with plasma homocysteine level higher than 
      150 microM. However, no difference was detected in brain Abeta levels or 
      deposition between the diet-treated and control group. As shown by western blot, 
      severe HHcy did not alter the steady state levels of proteins involved in AbetaPP 
      metabolism, either. These results demonstrate that this combination diet-induced 
      severe HHcy does not influence amyloidogenesis in vivo.
FAU - Zhuo, Jia-Min
AU  - Zhuo JM
AD  - Department of Pharmacology, Temple University School of Medicine, Philadelphia, 
      PA 19140, USA.
FAU - Pratico, Domenico
AU  - Pratico D
LA  - eng
GR  - R01 AG022512/AG/NIA NIH HHS/United States
GR  - AG-22512/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Alzheimers Dis
JT  - Journal of Alzheimer's disease : JAD
JID - 9814863
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Peptide Fragments)
RN  - 0 (amyloid beta-protein (1-40))
RN  - 0 (amyloid beta-protein (1-42))
RN  - AE28F7PNPL (Methionine)
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Alzheimer Disease/*etiology/genetics/*metabolism/pathology
MH  - Amyloid beta-Peptides/*metabolism
MH  - Amyloid beta-Protein Precursor/genetics
MH  - Animals
MH  - Cerebral Cortex/metabolism
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Gene Expression Regulation/genetics
MH  - Hippocampus/metabolism
MH  - Humans
MH  - Hyperhomocysteinemia/chemically induced/*complications/pathology
MH  - Methionine/administration & dosage
MH  - Mice
MH  - Mice, Transgenic
MH  - Peptide Fragments/*metabolism
MH  - Random Allocation
MH  - Vitamin B 12/administration & dosage
PMC - PMC3880572
MID - NIHMS540543
EDAT- 2010/06/18 06:00
MHDA- 2010/11/03 06:00
PMCR- 2014/01/04
CRDT- 2010/06/18 06:00
PHST- 2010/06/18 06:00 [entrez]
PHST- 2010/06/18 06:00 [pubmed]
PHST- 2010/11/03 06:00 [medline]
PHST- 2014/01/04 00:00 [pmc-release]
AID - VH850J00760631W5 [pii]
AID - 10.3233/JAD-2010-100171 [doi]
PST - ppublish
SO  - J Alzheimers Dis. 2010;21(1):133-40. doi: 10.3233/JAD-2010-100171.