PMID- 20555436
OWN - NLM
STAT- MEDLINE
DCOM- 20100927
LR  - 20220309
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 53
IP  - 6
DP  - 2010 Jun
TI  - Production and identification of wheat - Agropyron cristatum (1.4P) alien 
      translocation lines.
PG  - 472-81
LID - 10.1139/g10-023 [doi]
AB  - The P genome of Agropyron Gaertn., a wild relative of wheat, contains an 
      abundance of desirable genes that can be utilized as genetic resources to improve 
      wheat. In this study, wheat - Aegilops cylindrica Host gametocidal chromosome 2C 
      addition lines were crossed with wheat - Agropyron cristatum (L.) Gaertn. disomic 
      addition line accession II-21 with alien recombinant chromosome (1.4)P. We 
      successfully induced wheat - A. cristatum alien chromosomal translocations for 
      the first time. The frequency of translocation in the progeny was 3.75%, which 
      was detected by molecular markers and genomic in situ hybridization (GISH). The 
      translocation chromosomes were identified by dual-color GISH /fluorescence in 
      situ hybridization (FISH). The P genomic DNA was used as probe to detect the 
      (1.4)P chromosome fragment, and pHvG39, pAs1, or pSc119.2 repeated sequences were 
      used as probes to identify wheat translocated chromosomes. The results showed 
      that six types of translocations were identified in the three wheat - A. 
      cristatum alien translocation lines, including the whole arm or terminal portion 
      of a (1.4)P chromosome. The (1.4)P chromosome fragments were translocated to 
      wheat chromosomes 1B, 2B, 5B, and 3D. The breakpoints were located at the 
      centromeres of 1B and 2B, the pericentric locations of 5BS, and the terminals of 
      5BL and 3DS. In addition, we obtained 12 addition-deletion lines that contained 
      alien A. cristatum chromosome (1.4)P in wheat background. All of these wheat - A. 
      cristatum alien translocation lines and addition-deletion lines would be valuable 
      for identifying A. cristatum chromosome (1.4)P-related genes and providing 
      genetic resources and new germplasm accessions for the genetic improvement of 
      wheat. The specific molecular markers of A. cristatum (1.4)P chromosome have been 
      developed and used to track the (1.4)P chromatin.
FAU - Liu, Wei-Hua
AU  - Liu WH
AD  - National Key Facilities for Crop Gene Resources and Genetic Improvement, 
      Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 
      P.R. China.
FAU - Luan, Yang
AU  - Luan Y
FAU - Wang, Jing-Chang
AU  - Wang JC
FAU - Wang, Xiao-Guang
AU  - Wang XG
FAU - Su, Jun-Ji
AU  - Su JJ
FAU - Zhang, Jin-Peng
AU  - Zhang JP
FAU - Yang, Xin-Ming
AU  - Yang XM
FAU - Gao, Ai-Nong
AU  - Gao AN
FAU - Li, Li-Hui
AU  - Li LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Agropyron/*genetics
MH  - Chromosome Banding
MH  - Chromosomes, Plant/genetics
MH  - Genome, Plant/*genetics
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Poaceae/*genetics
MH  - *Translocation, Genetic
EDAT- 2010/06/18 06:00
MHDA- 2010/09/29 06:00
CRDT- 2010/06/18 06:00
PHST- 2010/06/18 06:00 [entrez]
PHST- 2010/06/18 06:00 [pubmed]
PHST- 2010/09/29 06:00 [medline]
AID - g10-023 [pii]
AID - 10.1139/g10-023 [doi]
PST - ppublish
SO  - Genome. 2010 Jun;53(6):472-81. doi: 10.1139/g10-023.