PMID- 20556365
OWN - NLM
STAT- MEDLINE
DCOM- 20101021
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 397
IP  - 7
DP  - 2010 Aug
TI  - Serum-based ALYGNSA immunoassay for the prostate cancer biomarker, total 
      prostate-specific antigen (tPSA).
PG  - 3151-4
LID - 10.1007/s00216-010-3827-0 [doi]
AB  - Prostate-specific antigen (PSA) is a serum glycoprotein overproduced by the 
      prostate in prostate cancer (> or = 4 ng/mL in the bloodstream). An immunoassay 
      for total PSA (tPSA) was developed using the ALYGNSA method to enhance capture 
      antibody orientation and a limit of detection of 0.63 ng/mL was reported, a limit 
      15-fold lower than a commercial tPSA ELISA assay. This ALYGNSA assay, however, 
      was performed using only buffer-based proteins and blocking agents (Mackness et 
      al., Anal Bioanal Chem 396:681-686, 2010). To improve the clinical application of 
      this system, a serum-based tPSA ALYGNSA was developed employing human serum. This 
      assay also resulted in a limit of detection of 0.63 ng/mL of tPSA protein. The 
      findings reported here provide support for the clinical application of this assay 
      for diagnosis, progression, treatment, and possible recurrence of prostate 
      cancer.
FAU - Mackness, Brian C
AU  - Mackness BC
AD  - Biochemistry Program, Department of Chemistry, College of Arts and Sciences, 
      University of Massachusetts Lowell, Lowell, MA 01854, USA.
FAU - McDonald, Melisenda J
AU  - McDonald MJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100617
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Biomarkers)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Biomarkers/blood
MH  - Humans
MH  - Immunoassay/*methods
MH  - Limit of Detection
MH  - Male
MH  - Prostate-Specific Antigen/*blood
MH  - Prostatic Neoplasms/*blood
EDAT- 2010/06/18 06:00
MHDA- 2010/10/22 06:00
CRDT- 2010/06/18 06:00
PHST- 2010/02/19 00:00 [received]
PHST- 2010/05/04 00:00 [accepted]
PHST- 2010/05/03 00:00 [revised]
PHST- 2010/06/18 06:00 [entrez]
PHST- 2010/06/18 06:00 [pubmed]
PHST- 2010/10/22 06:00 [medline]
AID - 10.1007/s00216-010-3827-0 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2010 Aug;397(7):3151-4. doi: 10.1007/s00216-010-3827-0. Epub 
      2010 Jun 17.