PMID- 20556878
OWN - NLM
STAT- MEDLINE
DCOM- 20101203
LR  - 20131121
IS  - 1618-131X (Electronic)
IS  - 1438-4639 (Linking)
VI  - 213
IP  - 3
DP  - 2010 Jun
TI  - Integration of Pseudomonas aeruginosa and Legionella pneumophila in drinking 
      water biofilms grown on domestic plumbing materials.
PG  - 190-7
AB  - Drinking water biofilms were grown on coupons of plumbing materials, including 
      ethylene-propylene-diene-monomer (EPDM) rubber, silane cross-linked polyethylene 
      (PE-X b), electron-ray cross-linked PE (PE-X c) and copper under constant 
      flow-through of cold tap water. After 14 days, the biofilms were spiked with 
      Pseudomonas aeruginosa, Legionella pneumophila and Enterobacter nimipressuralis 
      (10(6) cells/mL each). The test bacteria were environmental isolates from 
      contamination events in drinking water systems. After static incubation for 24 h, 
      water flow was resumed and continued for 4 weeks. Total cell count and 
      heterotrophic plate count (HPC) of biofilms were monitored, and P. aeruginosa, L. 
      pneumophila and E. nimipressuralis were quantified, using standard culture-based 
      methods or culture-independent fluorescence in situ hybridization (FISH). After 
      14 days total cell counts and HPC values were highest on EPDM followed by the 
      plastic materials and copper. P. aeruginosa and L. pneumophila became 
      incorporated into drinking water biofilms and were capable to persist in biofilms 
      on EPDM and PE-X materials for several weeks, while copper biofilms were 
      colonized only by L. pneumophila in low culturable numbers. E. nimipressuralis 
      was not detected in any of the biofilms. Application of the FISH method often 
      yielded orders of magnitude higher levels of P. aeruginosa and L. pneumophila 
      than culture methods. These observations indicate that drinking water biofilms 
      grown under cold water conditions on domestic plumbing materials, especially EPDM 
      and PE-X in the present study, can be a reservoir for P. aeruginosa and L. 
      pneumophila that persist in these habitats mostly in a viable but non-culturable 
      state.
FAU - Moritz, Miriam M
AU  - Moritz MM
AD  - Biofilm Centre, Department of Aquatic Microbiology, Faculty of Chemistry, 
      University of Duisburg-Essen, D-47057 Duisburg, Germany.
FAU - Flemming, Hans-Curt
AU  - Flemming HC
FAU - Wingender, Jost
AU  - Wingender J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Int J Hyg Environ Health
JT  - International journal of hygiene and environmental health
JID - 100898843
RN  - 0 (Elastomers)
RN  - 0 (Ethylenes)
RN  - 0 (Water Pollutants)
RN  - 0 (ethylene-propylene-diene-monomer)
RN  - 789U1901C5 (Copper)
RN  - 9002-88-4 (Polyethylene)
RN  - 9006-04-6 (Rubber)
SB  - IM
MH  - *Biofilms
MH  - Cold Temperature
MH  - Colony Count, Microbial
MH  - Copper
MH  - Elastomers
MH  - *Equipment Contamination
MH  - Ethylenes
MH  - In Situ Hybridization, Fluorescence
MH  - Legionella pneumophila/*growth & development/isolation & purification
MH  - *Manufactured Materials
MH  - Polyethylene
MH  - Pseudomonas aeruginosa/*growth & development/isolation & purification
MH  - Rubber
MH  - Sanitary Engineering
MH  - *Water Microbiology
MH  - Water Pollutants
MH  - *Water Supply
EDAT- 2010/06/18 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/06/18 06:00
PHST- 2010/06/18 06:00 [entrez]
PHST- 2010/06/18 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S1438-4639(10)00052-0 [pii]
AID - 10.1016/j.ijheh.2010.05.003 [doi]
PST - ppublish
SO  - Int J Hyg Environ Health. 2010 Jun;213(3):190-7. doi: 
      10.1016/j.ijheh.2010.05.003.