PMID- 20557373
OWN - NLM
STAT- MEDLINE
DCOM- 20101105
LR  - 20211020
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Print)
IS  - 0309-0167 (Linking)
VI  - 57
IP  - 1
DP  - 2010 Jul
TI  - TOPOIIalpha and HER-2/neu overexpression/amplification in Barrett's oesophagus, 
      dysplasia and adenocarcinoma.
PG  - 81-9
LID - 10.1111/j.1365-2559.2010.03580.x [doi]
AB  - AIMS: Topoisomerase IIalpha (TOPOIIalpha) and HER-2/neu are chromosome 17q genes 
      coamplified in various cancers; no data exist for Barrett's oesophagus (BO) and 
      BO adenocarcinoma (ADC). The aim was to investigate gene amplification and 
      protein overexpression of TopoIIalpha and Her-2/neu in non-dysplastic BO, 
      dysplastic BO, Barrett ADC, and chromosome 17 aneusomy. METHODS AND RESULTS: 
      Forty-four patients [18 BO, 13 BO with dysplasia (five low-grade dysplasia, eight 
      high-grade dysplasia) and 13 ADC in BO] were evaluated by immunohistochemistry 
      and fluorescence in situ hybridization (FISH). Genes (HER-2/neu and TOPOIIalpha) 
      and chromosome 17 were evaluated by FISH. Patients with BO, dysplasia and ADC 
      were compared. A significant association was found between TOPOIIalpha protein 
      overexpression and TopoIIalpha gene amplification, chromosome 17 aneusomy, 
      HER-2/neu gene amplification and HER-2 protein overexpression as well as between 
      HER-2 protein and HER-2/neu gene, TopoIIalpha gene and aneusomy for chromosome17, 
      and between the genes TOPOIIalpha and HER-2/neu. Gene amplification (HER-2/neu, 
      TOPOIIalpha), protein overexpression (HER-2/TOPOIIalpha), and chromosome 17 
      aneusomy were associated with dysplasia or ADC. Most BO patients showed no 
      amplification/overexpression/aneusomy for the above genes, proteins and 
      chromosome, with no differences between dysplasia and ADC. CONCLUSIONS: HER-2/neu 
      and TOPOIIalpha amplification/overexpression might discriminate between BO and 
      dysplasia/ADC. Chromosome 17 aneusomy is associated with dysplasia or ADC in BO.
FAU - Rossi, Elisa
AU  - Rossi E
AD  - 2nd Department of Pathology, University of Brescia-Spedali Civili, Brescia, 
      Italy.
FAU - Villanacci, Vincenzo
AU  - Villanacci V
FAU - Bassotti, Gabrio
AU  - Bassotti G
FAU - Donato, Francesco
AU  - Donato F
FAU - Festa, Andrea
AU  - Festa A
FAU - Cengia, Gianpaolo
AU  - Cengia G
FAU - Grisanti, Salvatore
AU  - Grisanti S
FAU - Cestari, Renzo
AU  - Cestari R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20100616
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
RN  - EC 5.99.1.3 (DNA Topoisomerases, Type II)
SB  - IM
MH  - Adenocarcinoma/diagnosis/*genetics/metabolism/*pathology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aneuploidy
MH  - Antigens, Neoplasm/*genetics/metabolism
MH  - Barrett Esophagus/diagnosis/*genetics/metabolism/*pathology
MH  - Chromosomes, Human, Pair 17/genetics
MH  - DNA Topoisomerases, Type II/*genetics/metabolism
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Diagnosis, Differential
MH  - Esophageal Neoplasms/diagnosis/*genetics/*pathology
MH  - Female
MH  - Gene Amplification
MH  - Gene Expression
MH  - *Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Receptor, ErbB-2/metabolism
MH  - Retrospective Studies
PMC - PMC2916224
EDAT- 2010/06/19 06:00
MHDA- 2010/11/06 06:00
CRDT- 2010/06/19 06:00
PHST- 2010/06/19 06:00 [entrez]
PHST- 2010/06/19 06:00 [pubmed]
PHST- 2010/11/06 06:00 [medline]
AID - HIS3580 [pii]
AID - 10.1111/j.1365-2559.2010.03580.x [doi]
PST - ppublish
SO  - Histopathology. 2010 Jul;57(1):81-9. doi: 10.1111/j.1365-2559.2010.03580.x. Epub 
      2010 Jun 16.