PMID- 20558233
OWN - NLM
STAT- MEDLINE
DCOM- 20101220
LR  - 20220317
IS  - 1638-6183 (Electronic)
IS  - 0300-9084 (Linking)
VI  - 92
IP  - 9
DP  - 2010 Sep
TI  - beta-sitosterol among other secondary metabolites of Piper galeatum shows 
      inhibition of TNFalpha-induced cell adhesion molecule expression on human 
      endothelial cells.
PG  - 1213-21
LID - 10.1016/j.biochi.2010.06.005 [doi]
AB  - A phytochemical investigation of the stems of Piper galeatum yielded one novel 
      amide, 1-(3'-hydroxy-5'-methoxycinnamoyl)-piperidine (5) along with four known 
      compounds, i.e. beta-sitosterol (1), cyclostachine-A (2), piperine (3) and 
      piperolein-B (4). The structures of all the five compounds, isolated for the 
      first time from this plant were unambiguously established on the basis of their 
      detailed spectral analysis. The structure of cyclostachine-A (2) was confirmed by 
      X-ray crystallographic studies and structures of known compounds were confirmed 
      by comparison of their physical and/or chemical data with those reported in the 
      literature, which were in complete agreement. Additionally, the crude extracts as 
      well as the isolated pure compounds were screened for their activity to inhibit 
      TNFalpha (tumour necrosis factor-alpha)- induced expression of cell adhesion 
      molecule ICAM-1 (intercellular adhesion molecule-1) on the surface of human 
      umbilical vein endothelial cells (HUVECs). Among all, beta-sitosterol (1) was 
      found to be the most active compound, which was taken for further studies. 
      beta-sitosterol also significantly inhibited the TNFalpha-induced expression of 
      VCAM-1 and E-selectin, which also play key role in various inflammatory diseases. 
      The functional correlation of cell adhesion molecules inhibition was assessed by 
      cell adhesion assay using human neutrophils. We found that beta-sitosterol 
      significantly blocks the adhesion of neutrophils to endothelial monolayer. To 
      elucidate the molecular mechanism of inhibition of cell adhesion molecules, we 
      investigated the status of nuclear transcription factor-kappaB (NF-kappaB) and 
      were able to establish that beta-sitosterol significantly blocked the 
      TNFalpha-induced activation of NF-kappaB.
CI  - Copyright (c) 2010 Elsevier Masson SAS. All rights reserved.
FAU - Gupta, Pankaj
AU  - Gupta P
AD  - Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi-110 
      007, India.
FAU - Balwani, Sakshi
AU  - Balwani S
FAU - Kumar, Sarvesh
AU  - Kumar S
FAU - Aggarwal, Neha
AU  - Aggarwal N
FAU - Rossi, Miriam
AU  - Rossi M
FAU - Paumier, Sarah
AU  - Paumier S
FAU - Caruso, Francesco
AU  - Caruso F
FAU - Bovicelli, Paolo
AU  - Bovicelli P
FAU - Saso, Luciano
AU  - Saso L
FAU - DePass, Anthony L
AU  - DePass AL
FAU - Prasad, Ashok K
AU  - Prasad AK
FAU - Parmar, Virinder S
AU  - Parmar VS
FAU - Ghosh, Balaram
AU  - Ghosh B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100615
PL  - France
TA  - Biochimie
JT  - Biochimie
JID - 1264604
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (E-Selectin)
RN  - 0 (Plant Extracts)
RN  - 0 (Sitosterols)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cells, Cultured
MH  - E-Selectin/metabolism
MH  - Endothelial Cells/*drug effects/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Neutrophils/cytology/drug effects
MH  - Piper/*chemistry
MH  - Plant Extracts/*pharmacology
MH  - Sitosterols/*pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 2010/06/19 06:00
MHDA- 2010/12/21 06:00
CRDT- 2010/06/19 06:00
PHST- 2010/02/26 00:00 [received]
PHST- 2010/06/04 00:00 [accepted]
PHST- 2010/06/19 06:00 [entrez]
PHST- 2010/06/19 06:00 [pubmed]
PHST- 2010/12/21 06:00 [medline]
AID - S0300-9084(10)00219-1 [pii]
AID - 10.1016/j.biochi.2010.06.005 [doi]
PST - ppublish
SO  - Biochimie. 2010 Sep;92(9):1213-21. doi: 10.1016/j.biochi.2010.06.005. Epub 2010 
      Jun 15.