PMID- 20560354
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20181201
IS  - 0001-6209 (Print)
IS  - 0001-6209 (Linking)
VI  - 50
IP  - 4
DP  - 2010 Apr
TI  - [Recombinant cIL-18-binding protein as an antagonist to cIL-18 enhanced PBMCs 
      secreting IFN-gamma].
PG  - 506-11
AB  - OBJECTIVE: Interleukin-18 (IL-18) is a proinflammatory cytokines that plays a key 
      role of immune regulation through activating the Th 1 cell and NK cell secreting 
      interferon-gamma (IFN-gamma). IL-18-binding proteins(IL-18BP) secreted in human 
      and mouse can antagonise the activity of IL-18. The homologue gene of IL-18BP 
      from fowlpox virus genome was predicted and identified of its expressed protein, 
      to use as an antagonist to IL-18 guiding disease. METHODS: The cIL-18BP gene was 
      isolated from the genome of fowlpox vaccine virus by PCR through a pair of 
      special primers and cloned into vectors pPICZalphaA, then expressed in yeast 
      GS115. The biologic activity of recombinant proteins binding with cIL-18 was 
      measured, inhibiting the activity of cIL-18 enhancing relative cells secreting 
      IFN-gamma was confirmed through ELISA. RESULTS: The cIL-18BP gene was cloned from 
      fowlpox virus and acquired high performance expression in yeast systems induced 
      with methanol identified by SDS-PAGE. Recombinant cIL-18BP purified can bind 
      specifically with recombinant cIL-18 determined by ELISA test. cIL-18BP can 
      antagonise the activity of cIL-18 with determining the IFN-gamma concentration 
      secreting in PBMCs and MSB1 cells stimulated by cIL-18 respectively. CONCLUSION: 
      The experiment indicates that recombinant cIL-18BP can inhibit the activity of 
      cIL-18 stimulating related immune cells secreting IFN-gamma. Deletion of the 
      cIL-18BP from virus may improve the safety and immunogenicity of fowlpox virus 
      vaccine.
FAU - Liu, Wenqiang
AU  - Liu W
AD  - Agricultural College of Liaocheng University, Prevention and Control Technology 
      Institute of Animal Disease in Liaocheng University, Liaocheng 252059, China. 
      liuwqsky@sina.com
FAU - Liu, Guiqin
AU  - Liu G
FAU - Qin, Xuling
AU  - Qin X
FAU - Wang, Guiying
AU  - Wang G
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Wu Xue Bao
JT  - Wei sheng wu xue bao = Acta microbiologica Sinica
JID - 21610860R
RN  - 0 (CD59 Antigens)
RN  - 0 (Carrier Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (Recombinant Proteins)
RN  - 0 (interleukin-18 binding protein)
RN  - 82115-62-6 (Interferon-gamma)
RN  - U202363UOS (Fenofibrate)
SB  - IM
MH  - Animals
MH  - CD59 Antigens/*immunology
MH  - Carrier Proteins
MH  - Fenofibrate/*antagonists & inhibitors
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/metabolism/*pharmacology
MH  - Interferon-gamma/*antagonists & inhibitors
MH  - Interleukin-18/metabolism/*physiology
MH  - Mice
MH  - Recombinant Proteins/genetics/metabolism
EDAT- 2010/06/22 06:00
MHDA- 2010/07/08 06:00
CRDT- 2010/06/22 06:00
PHST- 2010/06/22 06:00 [entrez]
PHST- 2010/06/22 06:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Wu Xue Bao. 2010 Apr;50(4):506-11.