PMID- 20560673 OWN - NLM STAT- MEDLINE DCOM- 20101021 LR - 20191210 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 58 IP - 13 DP - 2010 Jul 14 TI - Comparison of polyacetylene content in organically and conventionally grown carrots using a fast ultrasonic liquid extraction method. PG - 7673-9 LID - 10.1021/jf101921v [doi] AB - A rapid and sensitive analytical method for quantification of polyacetylenes in carrot roots was developed. The traditional extraction method (stirring) was compared to a new ultrasonic liquid processor (ULP)-based methodology using high-performance liquid chromatography-ultraviolet (HPLC-UV) and mass spectrometry (MS) for identification and quantification of three polyacetylenes. ULP was superior because a significant reduction in extraction time and improved extraction efficiencies were obtained. After optimization, the ULP method showed good selectivity, precision [relative standard deviations (RSDs) of 2.3-3.6%], and recovery (93% of falcarindiol) of the polyacetylenes. The applicability of the method was documented by comparative analyses of carrots grown organically or conventionally in a 2 year field trial study. The average concentrations of falcarindiol, falcarindiol-3-acetate, and falcarinol in year 1 were 222, 30, and 94 mug of falcarindiol equiv/g of dry weight, respectively, and 3-15% lower in year 2. The concentrations were not significantly influenced by the growth system, but a significant year-year variation was observed for falcarindiol-3-acetate. FAU - Soltoft, Malene AU - Soltoft M AD - Department of Food Chemistry, National Food Institute, Technical University of Denmark, Morkhoj Bygade 19, DK-2860 Soborg, Denmark. FAU - Eriksen, Morten Rosbjorn AU - Eriksen MR FAU - Trager, Anne Wibe Braendholt AU - Trager AW FAU - Nielsen, John AU - Nielsen J FAU - Laursen, Kristian Holst AU - Laursen KH FAU - Husted, Soren AU - Husted S FAU - Halekoh, Ulrich AU - Halekoh U FAU - Knuthsen, Pia AU - Knuthsen P LA - eng PT - Comparative Study PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Plant Extracts) RN - 25067-58-7 (Polyynes) SB - IM MH - *Agriculture MH - Chemical Fractionation/instrumentation/*methods MH - Daucus carota/*chemistry MH - Food, Organic/*analysis MH - Plant Extracts/analysis/*isolation & purification MH - Plant Roots/chemistry MH - Polyynes/analysis/*isolation & purification MH - Ultrasonics EDAT- 2010/06/22 06:00 MHDA- 2010/10/22 06:00 CRDT- 2010/06/22 06:00 PHST- 2010/06/22 06:00 [entrez] PHST- 2010/06/22 06:00 [pubmed] PHST- 2010/10/22 06:00 [medline] AID - 10.1021/jf101921v [doi] PST - ppublish SO - J Agric Food Chem. 2010 Jul 14;58(13):7673-9. doi: 10.1021/jf101921v.