PMID- 20560731
OWN - NLM
STAT- MEDLINE
DCOM- 20101019
LR  - 20100719
IS  - 1091-7691 (Electronic)
IS  - 0895-8378 (Linking)
VI  - 22
IP  - 9
DP  - 2010 Aug
TI  - Urban particulate matter in Beijing, China, enhances allergen-induced murine lung 
      eosinophilia.
PG  - 709-18
LID - 10.3109/08958371003631608 [doi]
AB  - It has been reported that ambient particulate matter (PM) in some large cities, 
      such as Beijing, China, causes adverse respiratory health effects. However, there 
      is currently no experimental report on the relationship between bronchial asthma 
      and urban PM (UPM) in northeast Asia. In this study, the microbial and chemical 
      substances adsorbed onto UPM collected in Beijing were excluded by heat-treatment 
      at 360 degrees C for 30 min. The effects of UPM or heated UPM (H-UPM) toward 
      allergic lung inflammation were compared in murine lungs to investigate the role 
      of organic substances. ICR mice were administrated intratracheally with the two 
      kinds of UPM and/or ovalbumin (OVA) 4 times at 2-week intervals. UPM and H-UPM 
      enhanced eosinophil recruitment induced by OVA in the alveoli and in the 
      submucosa of the airway, which has a goblet cell proliferation in the bronchial 
      epithelium. UPM and H-UPM synergistically increased Th-2 cytokines--interleukin 
      (IL)-4 and IL-13, eosinophil-relevant cytokines and chemokines, such as IL-5 and 
      monocyte chemotactic protein-3 (MCP-3), induced by OVA in bronchoalveolar lavage 
      fluid (BALF). The enhancing effects were much greater in UPM than in H-UPM. UPM 
      induced adjuvant effects on specific immunoglobulin E (IgE) and IgG1 production 
      by OVA. In an in vitro study using RAW264.7 cells, UPM increased the expression 
      of Toll-like receptor 2 (TLR2) mRNA, but not TLR4 mRNA. H-UPM caused no 
      expression of both TLR mRNAs. These results suggest that the aggravated lung 
      eosinophilia in UPM was due to activation of a Th2-associated immune response via 
      the activation of TLR2 by microbial materials. Chemical materials of air 
      pollutant origin contained in UPM, and inorganic components (elemental carbon, 
      mineral elements) in H-UPM, could also cause the aggravation.
FAU - He, Miao
AU  - He M
AD  - Department of Environmental and Occupational Health, College of Public Health, 
      China Medical University, Shenyang, China.
FAU - Ichinose, Takamichi
AU  - Ichinose T
FAU - Yoshida, Seiichi
AU  - Yoshida S
FAU - Nishikawa, Masataka
AU  - Nishikawa M
FAU - Mori, Ikuko
AU  - Mori I
FAU - Yanagisawa, Rie
AU  - Yanagisawa R
FAU - Takano, Hirohisa
AU  - Takano H
FAU - Inoue, Ken-ichiro
AU  - Inoue K
FAU - Sun, Guifan
AU  - Sun G
FAU - Shibamoto, Takayuki
AU  - Shibamoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
RN  - 0 (Air Pollutants)
RN  - 0 (Chemokines)
RN  - 0 (Particulate Matter)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Air Pollutants/*toxicity
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Cell Line
MH  - Chemokines/metabolism
MH  - China
MH  - Drug Synergism
MH  - Eosinophils/*drug effects/pathology
MH  - Gene Expression/drug effects
MH  - Hot Temperature
MH  - Intubation, Intratracheal
MH  - Lung/*drug effects/immunology/pathology
MH  - Macrophages/drug effects/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred ICR
MH  - Ovalbumin/administration & dosage/immunology
MH  - Particulate Matter/*toxicity
MH  - Pulmonary Alveoli/drug effects/metabolism/pathology
MH  - Pulmonary Eosinophilia/*chemically induced/immunology/pathology
MH  - RNA, Messenger/metabolism
MH  - Toll-Like Receptor 2/genetics/metabolism
MH  - Toll-Like Receptor 4/genetics/metabolism
EDAT- 2010/06/22 06:00
MHDA- 2010/10/20 06:00
CRDT- 2010/06/22 06:00
PHST- 2010/06/22 06:00 [entrez]
PHST- 2010/06/22 06:00 [pubmed]
PHST- 2010/10/20 06:00 [medline]
AID - 10.3109/08958371003631608 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2010 Aug;22(9):709-18. doi: 10.3109/08958371003631608.