PMID- 2056191
OWN - NLM
STAT- MEDLINE
DCOM- 19910729
LR  - 20220309
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 97
IP  - 1
DP  - 1991 Jul
TI  - Transforming growth factor-beta stimulates wound healing and modulates 
      extracellular matrix gene expression in pig skin: incisional wound model.
PG  - 34-42
AB  - Enhanced wound healing is elicited by exogenous administration of transforming 
      growth factor- beta 1 (TGF- beta 1) in split-thickness, excisional wounds in the 
      pig (Quaglino, Lab Invest 63:307-319, 1990). A study was designed to investigate 
      if the selective and localized effects of TGF-beta 1 found in the previous model 
      were dependent upon the type of wound or could be considered a more general 
      effect of the cytokine. Transdermal, sutured incisions in the pig were evaluated 
      by conventional histology and by in situ hybridization to reveal locally affected 
      gene expression of collagen, elastin, fibronectin, stromelysin, TGF- beta 1, and 
      basic fibroblast growth factor. Granulation tissue formation was markedly 
      enhanced at 6 d by a single injection of recombinant human TGF beta 1 at the time 
      of wound closure. Although granulation tissue was confined within the margins of 
      the incisional wound, prominent differences in hybridization signals were 
      observed between control and treated wounds. The stimulatory effect of TGF- beta 
      1 on granulation tissue formation was accompanied by a distinct enhancement in 
      cells expressing mRNA for several different extracellular matrix proteins 
      including collagens type I and III and elastin, whereas a single injection of 
      human recombinant TGF beta 1 (4 micrograms) at the wound site diminished the 
      expression of the neutral metalloprotease, stromelysin, and enhanced the 
      frequency and intensity of cells expressing TGF- beta 1. The data reinforce the 
      concept that TGF- beta 1 can act as a potent, auto-inductive modulator of 
      connective tissue remodeling during the repair process.
FAU - Quaglino, D Jr
AU  - Quaglino D Jr
AD  - Department of Pathology, Vanderbilt University School of Medicine, Nashville TN 
      37232-2561.
FAU - Nanney, L B
AU  - Nanney LB
FAU - Ditesheim, J A
AU  - Ditesheim JA
FAU - Davidson, J M
AU  - Davidson JM
LA  - eng
GR  - AG06528/AG/NIA NIH HHS/United States
GR  - GM 40437/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Fibronectins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
RN  - 9007-58-3 (Elastin)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Collagen/*genetics
MH  - Elastin/*genetics
MH  - Extracellular Matrix/drug effects/*metabolism
MH  - Fibronectins/genetics
MH  - Gene Expression/*drug effects
MH  - Granulation Tissue/drug effects
MH  - Matrix Metalloproteinase 3
MH  - Metalloendopeptidases/genetics
MH  - Swine
MH  - Transforming Growth Factor beta/genetics/*pharmacology
MH  - Wound Healing/*drug effects
EDAT- 1991/07/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1991/07/01 00:00
PHST- 1991/07/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1991/07/01 00:00 [entrez]
AID - 0022-202X(91)90199-Z [pii]
PST - ppublish
SO  - J Invest Dermatol. 1991 Jul;97(1):34-42.