PMID- 20562342
OWN - NLM
STAT- MEDLINE
DCOM- 20100830
LR  - 20211020
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 107
IP  - 27
DP  - 2010 Jul 6
TI  - Histaminergic responses by hypothalamic neurons that regulate lordosis and their 
      modulation by estradiol.
PG  - 12311-6
LID - 10.1073/pnas.1006049107 [doi]
AB  - How do fluctuations in the level of generalized arousal of the brain affect the 
      performance of specific motivated behaviors, such as sexual behaviors that depend 
      on sexual arousal? A great deal of previous work has provided us with two 
      important starting points in answering this question: (i) that histamine (HA) 
      serves generalized CNS arousal and (ii) that heightened electrical activity of 
      neurons in the ventromedial nucleus of the hypothalamus (VMN) is necessary and 
      sufficient for facilitating the primary female sex behavior in laboratory 
      animals, lordosis behavior. Here we used patch clamp recording technology to 
      analyze HA effects on VMN neuronal activity. The results show that HA acting 
      through H1 receptors (H1R) depolarizes these neurons. Further, acute 
      administration of estradiol, an estrogen necessary for lordosis behavior to 
      occur, heightens this effect. Hyperpolarization, which tends to decrease 
      excitability and enhance inhibition, was not affected by acute estradiol or 
      mediated by H1R but was mediated by other HA receptor subtypes, H2 and H3. 
      Sampling of mRNA from individual VMN neurons showed colocalization of expression 
      of H1 receptor mRNA with estrogen receptor (ER)-alpha mRNA but also revealed ER 
      colocalization with the other HA receptor subtypes and colocalization of 
      different subtypes with each other. The latter finding provides the molecular 
      basis for complex "push-pull" regulation of VMN neuronal excitability by HA. 
      Thus, in the simplest causal route, HA, acting on VMN neurons through H1R 
      provides a mechanism by which elevated states of generalized CNS arousal can 
      foster a specific estrogen-dependent, aroused behavior, sexual behavior.
FAU - Dupre, Christophe
AU  - Dupre C
AD  - Laboratory of Neurobiology and Behavior, The Rockefeller University, New York, NY 
      10065, USA.
FAU - Lovett-Barron, Matthew
AU  - Lovett-Barron M
FAU - Pfaff, Donald W
AU  - Pfaff DW
FAU - Kow, Lee-Ming
AU  - Kow LM
LA  - eng
GR  - T32 HD007430/HD/NICHD NIH HHS/United States
GR  - HD05751/HD/NICHD NIH HHS/United States
GR  - R37 HD005751/HD/NICHD NIH HHS/United States
GR  - MH38273/MH/NIMH NIH HHS/United States
GR  - R01 HD005751/HD/NICHD NIH HHS/United States
GR  - R01 MH038273/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100618
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Histamine Agonists)
RN  - 0 (Histamine Antagonists)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Histamine H1)
RN  - 0 (Receptors, Histamine H2)
RN  - 0 (Receptors, Histamine H3)
RN  - 4TI98Z838E (Estradiol)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Gene Expression
MH  - Histamine/*pharmacology
MH  - Histamine Agonists/pharmacology
MH  - Histamine Antagonists/pharmacology
MH  - Membrane Potentials/drug effects
MH  - Neurons/*drug effects/metabolism/physiology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Estrogen/genetics/physiology
MH  - Receptors, Histamine H1/genetics/physiology
MH  - Receptors, Histamine H2/genetics/physiology
MH  - Receptors, Histamine H3/genetics/physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sexual Behavior, Animal/physiology
MH  - Ventromedial Hypothalamic Nucleus/cytology
PMC - PMC2901472
COIS- The authors declare no conflict of interest.
EDAT- 2010/06/22 06:00
MHDA- 2010/08/31 06:00
PMCR- 2011/01/06
CRDT- 2010/06/22 06:00
PHST- 2010/06/22 06:00 [entrez]
PHST- 2010/06/22 06:00 [pubmed]
PHST- 2010/08/31 06:00 [medline]
PHST- 2011/01/06 00:00 [pmc-release]
AID - 1006049107 [pii]
AID - 201006049 [pii]
AID - 10.1073/pnas.1006049107 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12311-6. doi: 
      10.1073/pnas.1006049107. Epub 2010 Jun 18.