PMID- 20564442
OWN - NLM
STAT- MEDLINE
DCOM- 20110118
LR  - 20151119
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 90
IP  - 10
DP  - 2010 Aug 15
TI  - Insights into proteolytic processing of the major peanut allergen Ara h 2 by 
      endogenous peanut proteases.
PG  - 1702-8
LID - 10.1002/jsfa.4005 [doi]
AB  - BACKGROUND: The major peanut allergens are Ara h 1, Ara h 2 and Ara h 6. 
      Proteolytic processing has been shown to be required for the maturation process 
      of Ara h 6. The aim of this study was to examine whether Ara h 2 undergoes 
      proteolytic processing and, if so, whether proteolytic processing influences its 
      ability to bind human immunoglobulin E (IgE). RESULTS: Ara h 2 isolated from 
      peanut extract under conditions of protease inhibition revealed a single 
      additional peak for its two known isoforms (Ara h 2.01 and Ara h 2.02), 
      corresponding to a C-terminally truncated form lacking a dipeptide (RY). Ara h 2 
      isolated in the absence of protease inhibition, however, yielded two additional 
      peaks, identified as C-terminally truncated forms lacking either a dipeptide (RY) 
      or a single tyrosine residue. The IgE-binding capacity of the Ara h 2 truncated 
      forms was not altered. CONCLUSION: Ara h 2 undergoes proteolytic processing by 
      peanut proteases that involves C-terminal removal of a dipeptide. Hence Ara h 2 
      isolated from peanut extract is a complex mixture of two isoforms expressed by 
      different genes, Ara h 2.01 and Ara h 2.02, as well as truncated forms generated 
      by the proteolytic processing of these isoforms.
CI  - Copyright (c) 2010 Society of Chemical Industry.
FAU - Radosavljevic, Jelena
AU  - Radosavljevic J
AD  - Department of Biochemistry, Faculty of Chemistry, University of Belgrade, 
      Studentski Trg 12-16, Belgrade, Serbia. radosavljevic@chem.bg.ac.rs
FAU - Dobrijevic, Dragana
AU  - Dobrijevic D
FAU - Jadranin, Milka
AU  - Jadranin M
FAU - Blanusa, Milan
AU  - Blanusa M
FAU - Vukmirica, Jelena
AU  - Vukmirica J
FAU - Cirkovic Velickovic, Tanja
AU  - Cirkovic Velickovic T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (2S Albumins, Plant)
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Ara h 2 allergen, Arachis hypogaea)
RN  - 0 (Dipeptides)
RN  - 0 (Glycoproteins)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 42HK56048U (Tyrosine)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - 2S Albumins, Plant/chemistry/isolation & purification/*metabolism
MH  - Allergens/chemistry/isolation & purification/*metabolism
MH  - Antigens, Plant/chemistry/isolation & purification/*metabolism
MH  - Arachis/*chemistry
MH  - Dipeptides
MH  - Glycoproteins/chemistry/isolation & purification/*metabolism
MH  - Hydrolysis
MH  - Immunoglobulin E
MH  - Isomerism
MH  - Peptide Hydrolases/*metabolism
MH  - Plant Extracts/chemistry/metabolism
MH  - Plant Proteins/chemistry/isolation & purification/*metabolism
MH  - Tyrosine
EDAT- 2010/06/22 06:00
MHDA- 2011/01/19 06:00
CRDT- 2010/06/22 06:00
PHST- 2010/06/22 06:00 [entrez]
PHST- 2010/06/22 06:00 [pubmed]
PHST- 2011/01/19 06:00 [medline]
AID - 10.1002/jsfa.4005 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2010 Aug 15;90(10):1702-8. doi: 10.1002/jsfa.4005.