PMID- 20564564
OWN - NLM
STAT- MEDLINE
DCOM- 20101021
LR  - 20161125
IS  - 1612-1880 (Electronic)
IS  - 1612-1872 (Linking)
VI  - 7
IP  - 6
DP  - 2010 Jun
TI  - Effects of D-aspartate treatment on D-aspartate oxidase, superoxide dismutase, 
      and caspase 3 activities in frog (Rana esculenta) tissues.
PG  - 1459-66
LID - 10.1002/cbdv.200900331 [doi]
AB  - Although D-aspartate (D-Asp) has been recognized to have a physiological role 
      within different organs, high concentrations could elicit detrimental effects on 
      those same organs. In this study, we examined the D-aspartate oxidase (D-AspO) 
      activity and the expression of superoxide dismutase 1 (SOD1) and caspase 3 in 
      different tissues of the frog Rana esculenta after chronic D-Asp treatment. Our 
      in vivo experiments, consisting of intraperitoneal (ip) injections of D-Asp (2.0 
      micromol/g b.w.) in frogs for ten consecutive days, revealed that all examined 
      tissues can take up and accumulate D-Asp. Further, in D-Asp treated frogs, i) the 
      D-AspO activity significantly increased in all tissues (kidney, heart, testis, 
      liver, and brain), ii) the SOD1 expression (antioxidant enzyme) significantly 
      increased in the kidney, and iii) the caspase 3 level (indicative of apoptosis) 
      increased in both brain and heart. Particularly, after the D-Asp treatment we 
      found in both brain and heart (which showed the lowest SOD1 levels) a significant 
      increase of the caspase 3 expression and, vice versa, in the kidney (which showed 
      the highest SOD1 expression) a significant decrease of the caspase 3 expression. 
      Therefore, we speculate that, in frog tissue, D-AspO plays an essential role in 
      modulating the D-Asp concentration. In addition, exaggerated D-Asp concentrations 
      activated SOD1 as cytoprotective mechanism in the kidney, whereas, in the brain 
      and in the heart, where the antioxidant action of SOD1 is limited, caspase 3 was 
      activated.
FAU - Burrone, Lavinia
AU  - Burrone L
AD  - Dipartimento di Scienze della Vita, Seconda Universita degli Studi di Napoli, via 
      Vivaldi 43, I-81100 Caserta, Italy.
FAU - Di Giovanni, Marcello
AU  - Di Giovanni M
FAU - Di Fiore, M Maddalena
AU  - Di Fiore MM
FAU - Baccari, Gabriella Chieffi
AU  - Baccari GC
FAU - Santillo, Alessandra
AU  - Santillo A
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Chem Biodivers
JT  - Chemistry & biodiversity
JID - 101197449
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
RN  - EC 1.4.3.1 (D-Aspartate Oxidase)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Brain/enzymology/metabolism
MH  - Caspase 3/*metabolism
MH  - D-Aspartate Oxidase/*metabolism
MH  - D-Aspartic Acid/*administration & dosage
MH  - Injections, Intraperitoneal
MH  - Kidney/enzymology/metabolism
MH  - Myocardium/enzymology/metabolism
MH  - Rana esculenta
MH  - Superoxide Dismutase/*metabolism
MH  - Superoxide Dismutase-1
EDAT- 2010/06/22 06:00
MHDA- 2010/10/22 06:00
CRDT- 2010/06/22 06:00
PHST- 2010/06/22 06:00 [entrez]
PHST- 2010/06/22 06:00 [pubmed]
PHST- 2010/10/22 06:00 [medline]
AID - 10.1002/cbdv.200900331 [doi]
PST - ppublish
SO  - Chem Biodivers. 2010 Jun;7(6):1459-66. doi: 10.1002/cbdv.200900331.